| 1. Studies on the methodology of 18F-FDG cell binding of ovarian cancer SKOV3SKOV3 was a human ovarian cancer cell line. Operation, Radiotherapy and Chemotherapy were commonly used to treat ovarian cancer patients. Anatomic imaging, however, could not reflect therapeutic response in time. 18F-FDG was a structural analogue of glucose. 18F-FDG could be transported into the cell and metabolized. The binding efficiency of SKOV3 cell with 18F-FDG could reflect the glucose metabolism of tumor. It would be used to valuate the inhibitory effect of Radiotherapy and Chemotherapy on ovarian cancer cell line SKOV3.Objective: To establish a useful and stable methodology of 18F-FDG cell binding of ovarian cancer SKOV3Methods: Several binding conditions were tested. (1)The cell number was from 5×104 to5×106 cells/bottle.(2)The reaction time was from 20 to 120min. (3)The radioactivity of 18F-FDG was from 1.85 to 29.6KBq. (4)The glucose concentration was from 0 to 11.1mmol/ L.The count of CPM(B) in cells and CPM(F) in supernatant were measured by theγequipment .The binding efficiency of ovarian cancer cell line SKOV3 with 18F-FDG was calculated.Result: (1)When conditions were changed according above:①The binding efficiency was from (12.24±1.20)% to (40.60±5.02)% when the cell number was from 5×104 to 5×106 cells/bottle. The difference of binding efficiency was no significant between 5×104 and 1×105 cells /bottle group (P=0.270),while the difference of binding efficiency was significant between the others (P<0.05).②The binding efficiency was from (13.65±4.01)% to (24.27±2.80)% when reaction time was from 20 to 120min. The difference of binding efficiency was significant between 60 min and 120min group (P<0.05).③The binding efficiency was from (21.34±1.53)% to (29.62±0.38)% when radioactivity of 18F-FDG was from 1.85 to 29.6KBq. The difference of binding efficiency was no significant between 3.7KBq, 7.4 KBq and 14.8 KBq group (P<0.05).④.The binding efficiency was from (4.37±0.72)% to (22.98±1.69)% when glucose concentration was from 0 to 11.1mmol/L. The difference of binding efficiency was no significant between 5.55 and 11.1mmol/L group (P=0.131).While the difference of binding efficiency was significant between the others (P<0.05).(2)The basic conditions of 18F-FDG cell binding of ovarian cancer SKOV3: the cell number was 1×106 cells/bottle, the radioactivity of 18F-FDG was 3.7KBq, the glucose concentration was 0 mmol / L, the reaction time was 100 min. the binding efficiency was (25.45±1.66)%.Conclusions: The methodology of 18F-FDG cell binding of ovarian cancer SKOV3 was established successfully. It contributed to evaluate the effect of Radiotherapy and chemotherapy on inhibiting ovarian cancer cell line SKOV32. Experimental studies of 18F-FDG cell binding in early valuating the inhibitory effect of Taxinol on human ovarian cancer cell line SKOV3Objective: To study the feasibility of early valuating the inhibitory effect of Taxinol on ovarian cancer cell line SKOV3 with 18F-FDG. cell binding experiment.Methods: (1) To draw up cell growth curve of controlled group according to the optical density. (2) MTT was ultilized to measure the inhibitory rate on SKOV3 at different concertrations Taxinol at 12th, 24th, 36th, 48th, 60th and 72th (3) Morphology change of cells was observed with inverted microscope. Binding experiment was utilized to measure the inhibitory rate on SKOV3 at different concentrations Taxinol at 12th, 24th hour.Result: (1)The inhibitory rate of skov3 cell was correlated positively with the dose of Taxinol at the same time(12th～72th hour ), the Spearman correlation coefficient of dose and inhibitory rate was 0.910, 0.961, 0.937, 0.934, 0.856, 0.824, respectively (P=0.000). The inhibitory rate of SKOV3 cell was correlated positively with the time at the same concentration(100～1000nmol/L),the Spearman correlation coefficient of time and inhibitory rate was 0.968,0.982,0.960,0.966(P=0.000). (2)The cell size was increasing slightly and the cell amounts was decreasing in experimental group especially at 24th (3)①The difference of binding efficiency was significant between 1000nmol/L and controlled group at 12th hour(P<0.05). The difference of binding efficiency was no significant between 250 and 500nmol/L group(P=0.886)at 24th hour, while the difference of binding efficiency was significant between the others (P<0.05).②The difference of inhibitory rate of binding efficiency is no significant between 250 and 500nmol /L group at the same time. The difference of inhibitory rate of binding efficiency was significant between 12th hour and 24th hour group at the same concentration (P<0.05).Conclusions: SKOV3 cell was sensitive to Taxinol. 18F-FDG cell binding could be used to early evaluate the inhibitory effection of Taxinol on Ovarian Cancer SKOV3 cell.3. Experimental studies of 18F-FDG cell binding in early valuating the inhibitory effect of radiotherapy on human ovarian cancer cell line SKOV3Objective: To study the feasibility of early valuating the inhibitory effect of radiotherapy on Ovarian Cancer cell line SKOV3 with 18F-FDG cell binding experiment.Methods: (1) MTT was ultilized to measure the inhibitory rate on SKOV3 cell with different exposure dose of radiotherapy at 1th, 3th, 5th and 7th day. (2) Morphology Change of cells was observed with inverted microscope. Binding experiment was ultilized to measure the inhibitory rate on SKOV3 with different exposure dose of radiotherapy at 1th, 3th, 5th and 7th day . Result: (1)The inhibitory rate of SKOV3 cell was correlated positively with dose of radiotherapy, the Spearman correlation coefficient of dose and inhibitory rate was 0.787, 0.846, 0.905, 0.944, respectively at 1th, 3th, 5th and 7th day (P=0.000). When the dose of radiotherapy was 6Gy and 10Gy, the inhibitory rate of SKOV3 cell was correlated positively with the time at the same dose of radiotherapy, the Spearman correlation coefficient of time and inhibitory rate was 0.829, 0.969, respectively (P=0.000). When the dose of radiotherapy was 2Gy, the inhibitory rate of SKOV3 cell was not correlated with the time. The Spearman correlation coefficient of time and inhibition rate was 0.377(P=0.069).(2)The cell amounts was decreasing in 6Gy ,10Gy groups compared with controlled group. (3) The difference of binding efficiency was significant between 2Gy and 6Gy, 10Gy group at 1th day (P<0.05). The difference of binding efficiency was not significant between 6Gy and 10Gy group at 1th, 3th, 5th day(P>0.05).The difference of binding efficiency was significant between 2Gy, 6Gy, 10Gy group at 7th day (P<0.05).(4) The inhibitory rate of binding efficiency of SKOV3 cell with 18F-FDG was correlated positively with the dose of radiotherapy at the same time. The Spearman correlation coefficient of dose and inhibition rates was 0.800, 0.768, 0.787, 0.944 respectively at 1th, 3th, 5th and 7th day (P=0.000). When the dose of radiotherapy was 6Gy and 10Gy ,the inhibitory rate of binding efficiency of SKOV3 cell with 18F-FDG was correlated positively with the time at the same dose of radiotherapy, the Spearman correlation coefficient of time and inhibitory rate was 0.781, 0.940 respectively (P=0.000). When dose of radiotherapy was 2Gy, the inhibitory rate of binding efficiency of SKOV3 cell with 18F-FDG was not correlated positively with the time, the Spearman correlation coefficient of time and inhibition rate was 0.345(P=0.099). The difference of inhibitory rate of binding efficiency was not significant between 6Gy and 10Gy group at 1th, 3th, 5th day ( P=0.333, 0.459, 0.225 respectively). The difference of inhibitory rate of binding efficiency was significant between 2 Gy, 6 Gy, 10Gy group at 7th day (P<0.05).Conclusions: Ovarian cancer cell line SKOV3 was sensitive to Radiotherapy. 18F-FDG cell binding could be used to early evaluate the inhibitory effection of Radiotherapy on Ovarian Cancer SKOV3 cell.
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