| Tick is the second largest blood sucking arthropod in the world, and blood sucking process not only destroys the host(including human) skin causes pathogen infection, while tick is many extremely dangerous pathogens media, seems like: Borrelia burgdorferi, Anaplasma phagocytophilum, typhus fever etc. So it is of great significance to the research. Female tick would undergo great changes especially after mating and it can increase rapidly more than ten times. The blood provides the raw materials and energy to complete the spawning activities, so analyzing the marker molecule of different stages is meaningful for comprehensive understanding the special lifecycle of tick. Salivary gland is a very important tissue for tick, because it can promot a lot of blood sucking in the short period of tick. So to explore the molecular mechanism of salivary glands become importance method that prevention and treatment of tick.We adopted the generation sequencing technology(Illumina Hi Seq2500) to obtain the four stages of female tick(starvation period, unmated blood feeding period, mated half full period and full blood period) gene libraries. In order to find the specific genes expressed in different blood sucking period. At the same time, the data is used as the Database for the quantitative proteomic analysis of salivary gland. By the quality controlling, we got 16.39 Gb Clean Data. The percentage of Q30 bases in four samples are not less than 82.91%.We got 71586 Unigenes that owing N50=2109 through asembling reads from the clean data. Than, Unigenes were putted to seven different data bases named: Nr, swissprot, COG, KOG, GO, KEGG and Pfam and performing blast and as a result, 25619 Unigenes were annotated functions. As a result, we got a group that cluding 15414 annotated genes which coming from blasting of swissprot. Than we performed the GO analysis about different expression gene sets and described the differential expression genes that occurring four times of hyalomma asiaticum. FPKM vale of Unigene can show us which Unigene is expression and by comparing one Unigene’s FPKM values existing four periods,we can find the differential expression genes. At least, we got 6448 differential expression genes. Using i TRAQ reagents marking four different times salivary gland protein of Hyalomma asiaticum and among them, there were 617 proteins possessing fold Change not less than two times as well as at last two peptides(p<0.05) matching one Unigene, after analysis the different expression genes, we futher understood the metabolic about ticks, and giving a theoretical foundation for our next study. |
