Calcium Binding Protein S100A12 As A Mediator To Evaluate Severity And Curative Effect Of Severe Acute Pancreatitis

Objective The purpose of this study is to investigate the potential effects of calcium binding protein S100A12 on curative effect of severe acute pancreatitis.Methods Intraperitoneal injection of 50μg/kg caerulein for seven times (every interval time was an hour) and intraperitoneal injection of 10mg/kg lipopolysaccharide for once to establish acute pancreatitis mice models.160 specific pathogen free ICR female mice were randomly divided into the control group(Group A, normal saline), the mild group(Group B, caerulein), the severe group(Group C, caerulein+lipopolysaccharide) and the intervention group(Group D, S1O0A12 recombinant antibodies+caerulein+lipopolysaccharide), each group had 40 mice. We sampled the blood at 8 hours,12 hours, and 24 hours after the beginning of building animal models. In each period of time, we respectively detected the serum S100A12, amylase(AMY), C-reactive protein(CRP), interleukin(IL-1β, IL-6) and tumor necrosis factor(TNF-a) levels. And we observed and scored the pancreas histopathology of the mice. In addition, we used ELISA method to test patients’ plasma S100A12 level,81 patients with acute pancreatitis, whose plasma S100A12 level were compared, and we also used ROC curve to comprehensively analyze the clinical value of S100A12.Results In each same period of time compared with group C, serum AMY, CRP, IL-1β, IL-6, TNF-α, S100A12 levels of group D were significantly decreased(P<0.05). In each same period of time compared with group B, serum S100A12 concentration of group D was significantly decreased(P<0.05), and the pancreas histopathology were also much improved. The diagnostic performance of S100A12 was better than CRP concentration, Ranson and APACHE Ⅱ scoring systems.Conclusion These observations demonstrate that S100A12 recombinant antibodies were able to significantly reduce the severity of acute pancreatitis in mice. S100A12 may serve as a valid target of treatment in severe acute pancreatitis.