| Objective:Analysis of TGF- beta 1, Akt, m TOR expression in colorectal cancer and the relationship with clinical pathological parameters, discusses the role of the three in colorectal cancer occurrence, development, for the occurrence and development of colorectal cancer and pathological diagnosis and prognosis estimation to provide theoretical basis and objective basis.Methods:Surgical removal of paraffin embedded tissues from 123 cases of postoperative pathology in Lianyungang First People’s Hospital in January 2011 ~2014 in December was selected.123 patients in this study,male 75 cases, female 48 cases,age 29~87 years,Aged ≤55 years of 44 cases,> 55 years of 79 cases,mean age 58.6years; lymph node metastasis group 51 cases, 72 cases without metastasis; 28 cases of high differentiation, 46 cases of differentiation, 49 cases of low differentiated; according to the 2010 AJCC TNM staging for colorectal cancer in 45 cases,stage I and II stage in 66 cases, III- IV stage in 57 cases. Select 123 cases of normal colorectal tissue as a control.Adopted the technique of tissue microarray and immunohistochemical SP method detection of 123 cases of colorectal cancer and 123 cases of normal colorectal tissues in the expression of TGF-beta 1, Akt, m TOR, and analyze its relationship with clinical pathological characteristics of colorectal cancer.Processing data results using SPSS13.0 statistical software, and the results of the chi square test, TGF- beta 1, m TOR, Akt correlation comparison using Spearman Spearman test P<0.05(correlation coefficients) results with R said, the difference is statistically significant.Results:(1)The positive expression of TGF- β1 in normal colorectal and colorectal cancer was respectively 20.33%, 78.05%, a significant difference(P <0.01) statistically. In 123 cases of colorectal cancer, the expression of TGF-β1 and clinical stage, degree of differentiation and lymph node metastasis significantly correlated(P <0.05), and with the patient’s age, gender and tumor size(P> 0.05).(2)The positive expression of Akt in normal colorectal tissues was 10.57%, the positive expression of colorectal cancer tissue was 60.16%, both positive rate was significant difference(P <0.01), In 123 cases of colorectal cancer, the positive expression of Akt was significantly correlated with TNM stage and lymph node metastasis(P<0.01), and there was no significant correlation between the degree of differentiation, age, gender and age of patients(P>0.05).(3)The positive expression of m TOR in normal colorectal tissue was 6.50%,positive expression of colorectal cancer tissue was 59.35%, respectively, both the positive rate was significant difference(P <0.01). In 123 cases of colorectal cancer, the positive expression of m TOR was correlated with tumor differentiation degree, clinical stage and lymph node metastasis(P<0.01), and there was no significant correlation between tumor size and age and sex of patients(P>0.05).(4)With Spearman rank correlation analysis of the three markers pairwise comparison showed that in 123 cases of colorectal cancer expression and Akt expression of TGF-β1, TGF-β1 and m TOR no correlation between the expression of Akt and m TOR was a significant positive correlation(P <0.01).Conclusion:The positive expression rate of Akt, m TOR and TGF-β1 in colorectal cancer was significantly higher than that in normal colorectal mucosa, and the relationship between TNM stage, differentiation degree and lymph node metastasis with colorectal cancer in the clinical.They can be used as an effective biological indicators to determine the malignant degree of colorectal cancer. Expression of TGF- β1, Akt, m TOR, has a certain guiding significance to the clinical diagnosis, the treatment of colorectal cancer.Its mechanism may be related to the TGF- beta 1- HCT116EMT- PI3K/Akt/m TOR signaling pathways. |
PDF Full Text Request