Serum Chemotactic Factor Changes In Differentiated Thyroid Cancer Patients With Distant Metastases Or Dedifferentiation

Objective: This research aims to determine the correlation between chemotactic factor(CF) and dedifferentiation degree in DTC.Materials and methods: 76 DTC patients’ venous bloods were collected 26 male and 50 female, average age 41±13 from Shanghai 10 th People’s Hospital affiliated to Tongji University from January to May, 2015. These blood samples were divided into 2 categories according to metastasis or 131 I ablation effects, one category contains non-metastasis group(n=14), only neck lymph node metastasis group(n=47) and distal metastasis group(n=15); the other category contains single 131 I effective therapy group(n=63), multiple 131 I effective therapy group(n=9) and radioactive iodine resistant/refractory(RAIR) group(n=4). The Liquid microbeads floating protein chips(No. 171-AK99MR2, Huaying biochemical Science and Technology Co, Shanghai) were used to detect various CFs in these DTC blood samples through Bio-Plex Validation kit(Bio-Rad, USA). Those CFs include 6Ckine(CCL21), BCA-1(CXCL13), CTACK(CCL27), ENA-78(CXCL5), Eotaxin(CCL11), Eotaxin-2(CCL24), Eotaxin-3(CCL26), Fractalkine(CL3CL1), GCP-2(CXCL6), GM-CSF, Gro-α(CXCL1), Gro-β(CXCL2), I-309(CCL1), IFN-γ, IL-10, IL-1β, IL-2, IL-4, IL-6, IL-8(CXCL8), IP-10, IL-16, IP-10(CXCL10), I-TAC(CXCL11), MCP-1(CCL2), MCP-2(CCL8), MCP-3(CCL7), MCP-4(CCL13), MDC(CCL22), MIF, MIG(CXCL9), MIP-1α(CCL3), MIP-1θ(CCL15), MIP-13α,MIP-3β(CCL19), MPIF-1(CCL23), SCYB16(CXCL16), SDF-1α+β(CXCL12), TARC(CCL17), TECK(CCL25) and TNF-α.Results: Eotaxin-3, Fractalkine, GCP-2, Gro-α, IFN-γ, IL-1β, MCP-1, MDC, MIF, TECK and TNF-α were significant lower in metastasis group than non-metastasis group(P<0.05); comparing with neck lymph node metastasis group, MIP-1θ expression was significant higher and SCYB16 expression was significant lower in distal group(P=0.04); comparing with non-metastasis group, lower expression of Eotaxin, Eotaxin-3, Fractalkine, GCP-2, Gro-α, IFN-γ, MCP-4, MDC, MIF, TECK and TNF-α were found in neck lymph node metastasis group(P<0.05), while Eotaxin-3, Fractalkine, IL-16, MDC, SCYB16 and SDF-1α+β showed obviously lower in distal group(P<0.05). In multiple therapy group, MIP-1θshowed a significantly higher expression and SCYB16 expressed significantly lower than single therapy group(P=0.01). In comparison with the single therapy group, CTACK expression was obviously higher in RAIR group(P<0.05), while MPIF-1 and SDF-1α+β were significantly lower(P<0.05); in comparison with the multiple therapy group, CTACK and MIF were significantly higher but MPIF-1 and SDF-1α+β were much lower in RAIR group(P<0.05).Conclusions: SCYB16 may be associated with distal metastasis of DTC. For the RAIR patients, CTACK, MPIF-1 and SDF-1α+β were the definite CFs which showed significant different expression.