Research On The Correlation Between Cytokines INF-γ、IL-2、IL-4、IL-5、IL-10 And Tuberculous Pleurisy

Objective: To explore the cellular immunity in patients with tuberculous pleurisy and its important role in the occurrence and development of tuberculous pleurisy and to provide experimental basis for clinical diagnosis and prevention of this disease by measuring the content of cytokines of INF-γ,IL-2, IL-4, IL-5 and IL-10 in peripheral blood and pleural effusion in patients with tuberculous pleurisy through flow cytometry.Methods: The research objects were between 20 and 45 years old, divided into experimental group and control group, with 50 patients with tuberculous pleurisy in the experimental group and 15 patients with nontuberculous pleurisy and 15 healthy individuals in the control group. The content of cytokines of INF-γ, IL-2, IL-4, IL-5 and IL-10 in peripheral blood and pleural effusion was measured in the healthy patients, patients with tuberculous pleurisy and patients with non- tuberculous pleurisy through flow cytometry.Results:1 The comparison of the content of INF-γ, IL-2, IL-4, IL-5 and IL-10 in Peripheral blood in the healthy patients, Patients with tuberculous pleurisy and patients with non- tuberculous pleurisyThere was 42.19±17.96pg/mL of INF-γin the healthy individuals, 42.63±18.35pg/mL in the patients with tuberculous pleurisy and 38.43±18.80pg/mL in the patients with non- tuberculous pleurisy;there was 39.39±21.96pg/mL of IL-2 in the healthy patients, 41.31±19.23pg/mL in the patients with tuberculous pleurisy and 30.27±19.24pg/mL in the patients with non- tuberculous pleurisy; there was 49.5±20.75pg/mL of IL-4 in the healthy patients, 53.00±20.35pg/mL in the patients with tuberculous pleurisy and 49.2±18.35pg/mL in the patients with non- tuberculous pleurisy;there was 379±117.95pg/mL ofIL-5 in the healthy patients, 367±118.41pg/mL in the patients with tuberculous pleurisy and 410±119.35pg/mL in the patients with non- tuberculous pleurisy;there was 139±101.35pg/mL of IL-10 in the healthy patients, 137±102.47pg/mL in the patients with tuberculous pleurisy and 145±101.75pg/mL in the patients with non- tuberculous pleurisy. The statistical analysis showed that there was no obvious difference in the content of INF-γ, IL-2, IL-4, IL-5 and IL-10 in peripheral blood among the three groups of healthy patients, patients with tuberculous pleurisy and patients with non- tuberculous pleurisy or between any two groups of them(P>0.05)2 The comparison of the content of INF-γ, IL-2, IL-4, IL-5 and IL-10 in pleural effusion in the patients with tuberculous pleurisy and patients with non- tuberculous pleurisyThere was 47.77±27.67pg/mL of INF-γin pleural effusion in the patients with non- tuberculous pleurisy and a significant rise of almost 10 times(448.96±191.97pg/mL) was recorded in pleural effusion in the patients with tuberculous pleurisy, the statistical analysis of which showed very obvious difference(P<0.01); there was 70.07±24.95pg/mL of IL-2 in the patients with tuberculous pleurisy and 33.42±18.84pg/mL in the patients with nontuberculous pleurisy;there was 87.00±12.25pg/mL of IL-4 in the patients with tuberculous pleurisy and 48±15.55pg/mL in the patients with non- tuberculous pleurisy;there was 670±130.14pg/mL of IL-5 in the patients with tuberculous pleurisy and 385±119.35pg/mL in the patients with non- tuberculous pleurisy;there was 398±120.11pg/mL of IL-10 in the patients with tuberculous pleurisy and 146±189.75pg/mL in the patients with non- tuberculous pleurisy. The statistical analysis showed that the content of INF-γ, IL-2, IL-4, IL-5 and IL-10 in pleural effusion in the patients with tuberculous pleurisy was much higher than that in the patients with non- tuberculous pleurisy(P<0.01)Conclusion:1 The cytokines of INF-γ, IL-2, IL-4, IL-5 and IL-10 played a very important role in the occurrence and development of tuberculous pleurisy.2 The content of those cytokines obviously increased in pleural effusionin the patients with tuberculous pleurisy, indicating the enhanced local immune response. A reference for the diagnosis, identification and prognosis of tuberculous pleurisy could be provided through measuring the content of the cytokines of INF-γ, IL-2, IL-4, IL-5 and IL-10.3 IFN-γ is of great value for the diagnosis of tuberculous pleurisy.