| Background and significanceIn recent years, methicillin-resistant Staphylococcus aureus (MRSA) become a truly global challenge. It is a major human pathogen that causes a broad range of serious community-acquired and nosocomial diseases in humans,from minor skin infections to severe infections such as septicemia, septic shock, severe pneumonia, and et al.. In china it was first introduced in 1970s, then the separation rate increased rapidly. At present, MRSA has become a common pathogenic bacteria in hospital and community. It is not only with strong pathogenic ability and widely drug resistance spectrum, but also with a decreased trend year by year of sensitivity to variety of antimicrobial agents. As the result of one of the most serious infectious factors of public health. MRSA makes great difficulties for doctors to treat the patients who are infected by it. The MRSA has a high resistance rate to most of the antibiotics used in clinic currently and is multidrug-resistant β-lactam antibiotics, aminoglycosides, quinolones, macrolides etc. there are few antibiotics could choosed in clinic now.The increasing prevalence of MRSA and its ability to spread in hospitals and communities,has posed a major challenge for infection control. Knowledge of epidemic and the drug-sensitivity characteristics of MRSA clones can help the development of effective strategies to aid in controlling dissemination, optimizing treatment, and revealing the mode of pathogenicity. Monitoring the prevalent MRSA clones through current genotyping techniques is a crucial step to reveal relatedness among isolates.In FuYang, there is limited detailed data on Staphylococcus aureus especially the MRSA obtained from both inpatient and outpatient populations, and little attention has been paid to the molecular epidemiology of this pathogen. Accordingly,this study was conducted to better understand MRSA infections and to characterize the isolates by a variety of molecular typing techniques, eventially provide an overview of the MRSA. It will be great help for medical staff and the department of Hospital Infection Management to be more familiar with the epidemiology and drug susceptibility characteristics of MRSA, also be helpful to the workers in the Centers for Disease Control (CDC) to grasp the molecular characteristics of MRSA strains in our local area.ObjectTo investigate the genotype,epidemic characteristics and resistance of MRSA in Fuyang area.MethodStaphy-lococcus aureus were collected from kinds of specimens in two hospital in Fuyang area between November 2013 and October 2014. pvl assay, MLST, spa, SCCmec typing and PFGE typing were carried out with all the MRSA isolates. Drug-sensitivity test was finished by the vitek2 compact automatic system.Results44 MRSA strains were involved in our study.which was 16.4% of the Staphylococcus aureus.Two involved hospitals hold different epidemic clones: ST59-t437-SCCmecIV in Orthopedics & Traumatology Hospital and ST5-t311-SCCmec Ⅱ in the other hospital.The homology of the strains collected from the people’s hospital was high.6.8% (3/44) isolates were positive for PVL. All MRSA isolates were resistant to the β-lactam antibiotics, more than 85.0% resistant to Erythromycin and Clindamycin and were completely susceptible to Vancomycin, Linezolid and Tigecycline.ConclusionNow the detectable rate of MRSA in Fuyang is low relatively, and generally speaking,the prevalence of MRSA in FuYang is sporadic. the drug resistance of MRSA is serious, and the phenomenon of multiple resistance is very common. the result of the drug-sensitive is associated with the gentype of the isoaltes. |
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