| Backgrounds:Primary biliary cirrhosis(PBC) is now known as primary biliary cholangitis(PBC), is a autoimmune liver disease characterized by chronic progressive cholestatic. The main diagnostic indicator for PBC patients is anti-mitochondrial antibody(AMA) positive, but studies have found that about 5%-10% of patients with PBC serum AMA negative, and the current liver biopsy for pathological diagnosis is the gold standard of clinical diagnosis for PBC patients, but at high risk for liver biopsy limit its extensively in clinical. Recent studies have found that B cell infiltration in patients of AMA-positive and negative with varying degrees of liver tissue, suggesting that in the pathogenesis of PBC, B cells have a regulatory role for specific portal areas damage.micro RNA(mi RNA) is a class of non-coding RNA found in recent years which regulating the expression of the target gene in the post-transcriptional level. micro RNA controlled various immune cell subsets differentiation and immune function,involved in the regulation of B cells, different stages of development. What’s more, recent studies have found that abnormal expression of micro RNA exist in both peripheral blood and liver tissue of PBC patients.Objectives:In this study, by testing micro RNA spectrum of peripheral blood B cells with each period of PBC patients and healthy controls,We select specific expression altered micro RNA or micro RNA expression profiling,and reveal whethe micro RNA is related to progression of the PBC and clear the possible mechanisms between them.Methods:This study collected 32 cases of peripheral blood of patients with PBC which hospitalized in Hepatopancreatobiliary Medicine of the First Hospital of Jilin University form September 2013 to September 2015 and no sex and age differences in choice of healthy controls four cases. We extracted B cells from the peripheral blood B cells of both group and frozen at-80℃, and then the above sample was sent to Shanghai Kangcheng Sheng Company Performed micro RNA microarray. Differentially expressed of micro RNA defined as the false discovery rate(FDR) less than 0.05, fold change greater than 2.Results:This study includes a total of 32 patients with PBC, including pathological stage I of 7patients, II of 13 patients, III of the 6 patients, IV of 6 patients. Compared with the healthy control group, a total of 558 kinds of micro RNA expressed abnormal in peripheral blood B cells of PBC patients. Stage I and stage II, stage III, IV Contrast, stage II and shage III, IV,contrast, stage III of IV contrast,there are different types of micro RNA abnormal expression.Compared with healthy controls,hsa-mi R-133 b expressed significantly abnormal in peripheral blood B cells with each shage of PBC patients(fold change> 3713, P<0.01;fold change> 2771, P<0.01; fold change >2960, P<0.01; fold change >3305, P<0.01), and the expression was no significant difference in peripheral blood B cells for each shage of PBC patients(P=0.39). hsa-mi R-4770 and hsa-mi R-148a-3p expressed abnormal only in peripheral blood B cells of I stage of PBC patients, and the expression was significantly upregulated. There were two micro RNAs(hsa-mi R-223-3p, hsa-mi R-21-5p), which were differentially expressed in the four stage and decreased gradually with pathological stage. C were selected and micro RNA were verified by real-time PCR in 8 healthy controls and 37 PBC patients and the results were consistent with the results of the array.Conclusion:(1) hsa-mi R-4770 and hsa-mi R-148a-3p expressed abnormal only in peripheral blood B cells of I stage of PBC patients, and the expression was significantly upregulated.(2) hsa-mi R-223-3p, hsa-mi R-21-5p decrease gradually with the aggravation of PBC stage, indicating that they may involve in the pathogenesis of PBC. |
