The Preliminary Study On Expression Of Peripheral Blood Th17Cell And IL-17in Primary Biliary Cirrhosis

ObjectiveThis study is about three groups of patients with primary biliary cirrhosis(PBC) and chronic hepatitis B(HBV) and health controls(HC) which were performed inperipheral blood and liver specimens Th17/CD4+T cells ratio and the expression levels of IL-17,Simultaneously analyzed the correlation between the serum levels of IL-17and the markers of liver injuryed gamma-glutamyl transferase (GGT)、alkaline phosphatase(ALP),compared with the correlation of peripheral blood、liver tissue Th17/CD4+T cells and serum IL-17.With the result of liver biopsy analy the function and clinical significance of Th17/IL-17in PBC patients.Methods1Collect the research object:26cases of PBC patients;20cases of HBV patients and20healthy control group.2The ratio of Thl7cells/CD4+T cell in the peripheral blood and liver tissues were detected by flow cytometry(FCM).3The levels serum of IL-17were measured by enzyme-linked immunosorbent assay(ELISA), compared the correlation between the levels of IL-17and GGT、ALP.4Compared the correlation of the ratio of Th17/CD4+T cell in the peripheral blood、liver tissues and the serum of IL-17.5PBC patients were divided into two groups by liver biopsy results, respectively PBC Ⅰ-Ⅱ and PBC Ⅲ-Ⅳ,compared the differences of Th17/CD4+T cell in the peripheral blood、liver tissues and the serum of IL-17in PBC patients. Results1PBC、HBV and HC Th17/CD4+T cells in the peripheral blood levels were (36.53±8.38)、(29.85±14.27)、(19.51±5.34) respectively, the three groups have statistical significance of difference(P<0.05).PBC patients’ Th17/CD4+T cells in the peripheral blood are higher than that in HBV and HC (P<0.05). HBV patients’ Thl7/CD4+T cells in the peripheral blood are higher than that in HC (P<0.05).2PBC、HBV and health controls(HC) Th17/CD4+T cells in the liver tissue were (44.44±15.13).(28.62±11.68)%(18.78±5.146) respectively, the three groups have statistical significance of difference(P<0.05).PBC patients’ Th17/CD4+T cells in the pliver tissue are higher than that in HBV and HC (P<0.05). HBV patients’Th17/CD4+T cells in the liver tissue are higher than that in HC (P<0.05).3The serum level of IL-17in the three groups were measured by EL AS A, the serum of level of IL-17in PBC、HBV and HC were (28.37±5.37)、(20.79±4.22)、(17.13±4.07) respectively, the three groups have statistical significance of difference(P<0.05). PBC patients’IL-17level in the peripheral blood are higher than that in HBV and HC (P<0.05). PBC patients’IL-17in the peripheral blood are higher than that in HC (P<0.05).4PBC、HBV and HC ALP、GGT in the peripheral blood levels were (346.1±216.59)、(95.15±37.03)、(78.25±19.31) respectively, PBC patients’ALP. GGT level in the peripheral blood are higher than that in HBV and HC (P<0.05). The level of ALP、GGT in HBV and HC are similar(P>0.05).5The resulted showed PBC patients with the serum level of IL-17and ALP、GGT were positively correlated (P<0.05). In the peripheral、liver tissue of Thl7/CD4+T cells and ALP、GGT are not correlated (P>0.05).6PBC Patients have no correlation between Th17/CD4+T cells and IL-17(p<0.05).7PBC Ⅰ-Ⅱ and PBC Ⅲ-Ⅳ patients’ Th17/CD4+T in the peripheral blood are (31.17±8.3),(38.91±7.45) respectively, The level of Th17/CD4+T in the liver tissue are (34.24±10.35).(48.99±14.89) respectively, They all are different(P<0.05). The level of IL-17in the peripheral blood are (27.05±4.14)、(28.77±5.56) respectively, there is no different. ConclusionIn this experiment, we found that PBC patients’ peripheral blood, liver tissue Th17/CD4+T cells and serum IL-17is higher than that of HBV HC. HBV patients’ peripheral blood and liver tissue Th17/CD4+T cells and serum IL-17is higher than that of HC. PBC patients’ IL-17and ALP, GGT are correlated; No correlation between PBC patients’Th17/CD4+T cells in peripheral、hepatic tissues and serum IL-17, maybe influenced by cytokines、chemokines and liver microenvironment. Compared PBC I-II and PBCⅢ-Ⅳ, the peripheral blood and liver tissue in Th17/CD4+T cells are higher than the former. Th17cells and the secretion of pro-inflammatory cytokines IL-17play an important role in pathogenesis and immune inflammation and fibrosis of liver in PBC.