The Study Of Araneus Ventricosus Powder On Toxicity And Anti-inflammatory

Spiders are one of the most common species in China, and are widely distributed in China. Araneus ventricousus is a species of spider which is widely distributed in our country. With the rapid development of molecular biology and other technologies, the research on spiders is getting deeper and deeper. This has also attracted more and more interest on spider. But the most of the research on spiders are about this kind of Selenocosmia huwena with highly toxicity. Study on Araneus ventricosus is less and less, At present, the most researches of it were about Spider silk and venom components. But about its toxicological research and its anti-inflammatory activity has not been reported.In the study of individual toxicity, we researched acute toxicity and the maximum tolerated dose with the healthy Kunming mice by intragastric administration. Then these mice were through the continuous irrigation stomach to give till a month. The changes of body weight, organ coefficient and blood biochemical indexes were measured to detect the subacute toxicity.At last, the genetic and reproductive toxicity of the spider were studiedaccording to study genetic and reproductive toxicity of the spider by micronucleus of bone marrow and sperm abnormality in the way of intragastric gavage., Tthe result of subacute toxicity tests showed that compared with the control group, the changes of experimental group in weight, organ coefficients, blood cells and biochemical indexes of blood were not significantly different( P>0.05). These results indicated that the spider powder was innoxious substance, and it can apply to other study. The studies about g Genetic and reproductive toxicity showed that micronucleus and the sperm abnormality rate of mice were not significantly different compared withfrom blank control group( P>0.05). These result showed that the Araneus ventricosus had no cytotoxicity and had not effect on the reproductive and genetic in mice.L929 cell was used to study the cytotoxicity in vitro of the Araneus ventricosu powder. Toxicity which foreign substances to cells was reflected by their survival rate. Experimental results showed that Araneus ventricosus spiders had no effect on the proliferation of L929 cell(P>0.05).In order to explore the anti-inflammatory activity of Araneus ventricosus powder, Experimental we studiedy on acute ear swelling inflammation model and capillary vessel permeability in mice, study the anti-inflammatory effect with on inflammation of different concentrations of Araneus ventricosus powder on the inhibitory effect of individual miceby acute ear swelling inflammation model and capillary vessel permeability of mice. And Tthe experimental results of the vascular permeability showed that the increase of Araneus ventricosus powder could induced acute ear swelling andreduce the acetic acid induced mouse peritoneal capillary permeability. In this experiment, CCK-8 colorimetric method was used to detect different concentrations of Araneus ventricosus powder on LPS stimulated RAW264.7 cells. And we studied the anti-inflammatory effect of Araneus ventricosus powder on inflammatory cells by detecting the expression of IL-6 and TNF- alpha. The result showed that there was no significant effect of on the proliferation of Araneus ventricosus powder on inflammatory cells. But the expression of the IL-6and TNF- α in RAW264.7 cells wasinduced by IL-6 and TNF- α has inhibitedory effect. And c Compared with the LPS group, it had significant difference(P<0.05). Under this concentration conditions the result showed that Araneus ventricousus powder had no toxic, and. But it had better inhibitory effect to the expression of IL-6 and TNF-α in RAW264.7 cells induced by LPS. These results showed that the Araneus ventricousus powder has anti-inflammatory effect.