| Background:Gastric cancer is one of the most common malignant tumors in the world. The incidence of gastric cancer is lower in developed countries than in developing countries. The prognosis of Gastric cancer usually depends on the early detection and treatment of malignant tumor characteristics such as invasion and metastasis, which may cause treatment failure. Therefore, the exploration of initiation and progression mechanisms may improve the early diagnosis and treatment efficacy of Gastric cancer. Recent evidence has shown that aberrant EMT activation and HIF-1α/p53 pathway in the human stomach are closely associated with gastric carcinogenesis and tumor progression. Notch signaling pathway plays an important role in the processes of cell-fate determination, including differentiation and proliferation. Moreover, deregulated Notch-1 receptor expression could induced a growing number of human tumors. The Fu-Zheng-Xiao-Jia Recipe has been used clinically for several years, but the underlying mechanism is unknown.Objective:To observe the clinical efficacy and side effects of Fu-Zheng-Xiao-Jia Recipe combined with chemotherapy on advanced gastric cancer, and investigate the effects of Fu-Zheng-Xiao-Jia Recipe combined with 5-Fu on the expression of hypoxia-inducible factor-1α(HIF-1α), Notchl and p53 in cultured gastric cancer SGC-7901 cells.Methods:44 cases of patients with advanced gastric cancer were randomly divided into the control group and the treatment group,22 cases in each group. The control group was treated with oxaliplatin and tegafur, the treatment group received oxaliplatin, tegafur and Fu-Zheng-Xiao-Jia Recipe. The total efficiency, clinical symptoms, quality of life and adverse reactions were observed. We detected different concentrations of 5-Fu(10,20,50,100,200 μg/mL) on the cell proliferation of gastric cancer SGC-7901 cells by CCK-8 assay. The cells were collected after treated with Fu-Zheng-Xiao-Jia Recipe and 5-Fu. Real-time quantitative RT-PCR, western blot and immunofluorescence were employed to detect the gene and protein expression of HIF-1α and p53.Results:The total efficiency of the treatment group was higher than the control group,but there are no significant statistical difference between the two groups(P<0.05). The patients’quality of life improved significantly compared with the control group(P<0.05), and side effects of the treatment group was significantly reduced(P<0.05).5-Fu could time- and dose-dependently inhibited the proliferation of SGC-7901 cells. We found higher expression of Notchl, HIF-1α/VEGF and p53 in human gastric cancer SGC-7901 cells. The Fu-Zheng-Xiao-Jia Recipe combined with 5-Fu could inhibit the expression of HIF-1α and p53.Conclusion:The Fu-Zheng-Xiao-Jia Recipe can enhance the effect of chemotherapy for advanced gastric cancer patients, improve the quality of life and reduce side effects of chemotherapy. The Fu-Zheng-Xiao-Jia Recipe combined with 5-Fu could simultaneously inhibit the expression of HIF-1α/VEGF and p53 in human gastric cancer SGC-7901 cells, which may be one of the mechanisms of tumor suppression. |
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