Aloe-Emodin Mediated Photodynamic Therapy Inhibits Proliferation Migration Invasion And Improves Apoptosis In Human Breast Cancer Cells

Objective To determine the effects of photodynamic therapy (PDT) with aloe emodin(AE) as photosensitizer on the proliferation、igration、 invasion and apoptosis in breast cancer cells and investigate the underlying mechanism.Methods Cell cytotoxic effect was measured by WST-8 assay. Cellular apoptosis was detected by Hoechst nuclear staining, flow cytometry. Migration and invason of cell were detected by Transwell. Cytoskeleton disorganization were observed by Phalloidin-TRITC staining. The level of reactive oxygen species (ROS) were observed by DCFH-DA staining. Mitochondrial membrane potential was tested by JC-1 staining. The expression ofCaspase-9、BIP、Chop、VEGF、Nrf2、 MMP2 MMP9、Caspase-3 and Bcl-2 was assessed by Western blotting.Results Analysis of cell proliferation evidenced that there was a dramatical depression after photodynamic treatment with a series of aloe-emodin concentration and light doses showed.We observed changes apoptosis and demonstrated that the mechanism of apoptosis was involved of mitochondrial and endoplasmic reticulum death pathyway.The capacity of adhesion,migration and invasion of breast cells were measured usingWST8 and transwell assay and demonstrated that AE-PDT significant inhibited adhesion, migration and invasion of MCF-7cells,the expression of MMP2,MMP9,VEGF and Nrf2 demonstrated that the metastasis was related to oxidative stress.Analysis of changes in cytoskeleton components(F-actin) evidenced cytoskeleton disorganization after treatment with AE-PDT. Taken together, the present results indicated that PDT with aloe-emodin effectively suppressed cancer development in MCF-7cells, suggesting AE its potential as a new photosensitizer in PDT has the potential to become mainstream of cancer treatment...