| Objective:To investigate the effect of fluoxetine and desipramine on the cerebral frontal ischemic mice model of stroke. Discuss if desipramine has the effects on brain neural functional recovery and the effects compared to fluoxetine.Methods:(1) A photochemical model had been used in our experiment:the mouse was injected with Chloral Hydrated with a concentration of 4% and a Rose Bengal with a concentration of 1%.when deep anaesthesia was reached, Firmly fix the head with a head holder, be careful not to damage its neck, shave the mouse scalp with an electric razor, dissect scalp in a cut of 1-1.5cm vertically and keep the skull exposed. After ten minutes since the Rose Bengal had been injected, Identify the bregma and lambda, find the point had been positioned. Mark the points and put a cold light illuminator in close contact with the skull surface to avoid light scattering but pay attention not to exert pressure on it. After the cold light illuminator have been lasted for 10 minutes, then stich the cut remove the mouse from the stereotaxic apparatus and put it on a pre-warmed heating pad until it is fully awake, then return it to its cage.(2) The mice were randomly and evenly divided into:normal group, solvent control group, fluoxetine (10mg/kg) drug control group, desipramine (10mg/kg) drug control group, the normal mice and the solvent control group mice were given equivalent dose of saline of 0.9% as drug groups. The injection will be lasting for 28 days in antraperitoneal way. The Behavior Score, Rota-rod Test and Grip Forced Test will be investigated on the 1th,7th,14th,21th,28th day after the surgery. Animals were sacrificed on the 29th day of surgery. Before TTC staining, freshly dissected brain was positioned in a brain slicer and sectioned into 2 mm thick slices, TTC labels intact tissue in red whereas the infarcted region appears pale, which allows a precise measurement of the infarct area. The infarct area will be calculated by computer software.Result:(1) In the Rota-rod Test, for the results of the 28th day after the surgery: compared with the solvent control group, desipramine (10mg/kg) group rotating rod time increased notably and had statistical significance(P<0.05); compared with the fluoxetine group, the desipramine (10mg/kg) group mice rotating rod time decreased notably, had statistical significance (P<0.01).(2) In the Grip Forced Test, for the results of the 21th day after the surgery:compared with the solvent control group, the desipramine (10mg/kg) group mice strongest grip strength decreased notably, which had statistical significance (P<0.05); for the results of the 28th day after the surgery:desipramine (10mg/kg) group mice strongest grip strength decreased greatly, which had statistical significance (P<0.001); the fluoxetine (10mg/kg) group mice strongest grip strength decreased notably, had statistical significance (P<0.05)(3) In the behavior score test, for the results of the 28th day after the surgery, compared with the solvent control group, desipramine (10mg/kg) group mice behavior score decreased remarkably and had statistical significance (P<0.05); compared with the solvent control group, compared with fluoxetine control group, desipramine (10mg/kg) group the behavior score decreased remarkably and had statistical significance (P<0.05).(4)The infarct area calculation, the mice were sacrificed on the 29th day of surgery: compared with the solvent control group, fluoxetine (10 mg/kg) group infarct area was remarkably alleviated and a great significant deference (P<0.01) compared with the solvent control group, desipramine (10 mg/kg) group infarct area was remarkably alleviated and significant deference (P<0.01).Conclusion:Desipramine has neural function recovery and cerebral protective effects on the mice of a stroke model. Fluoxetine may have the neural function recovery effect on the mice of a stroke model. |
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