The Role Of Interleukin 33,38 In The Pathogenesis Of Psoriasis Yulgaris And Henoch-Schonlein Purpura

Part1Expression of IL-33,IL-38 in peripheral blood of patients with psoriasis vulgaris.Objective:To detect the expression of IL-33, IL-38 in peripheral blood of patients with psoriasis vulgaris.Methods:Select peripheral blood from 63 cases of PV patients,with 22 cases in progressive,20 cases in static, and 21 cases in paracmasis, together with 32 healthy controls. Competitive inhibition enzyme linked immunosorbent assay was used to detect the levels of serum IL-33 and IL-38.Results:The expression of serum IL-33 were significant higher in progressive (69.08± 11.54pg/ml) of PV than in static (58.18±16.38pg/ml), paracmasis (54.65± 14.81pg/ml) and healthy control (60.85±14.llpg/ml), all P<0.05. there were no significants between the group in static, paracmasis of PV and healthy control(p>0.05).The levels of serum IL-38 were significant higher in progressive(40.70+9.17pg/ml), static(84.54±17.59pg/ml) and paracmasis(78.88±14.32pg/ml) of PV than in healthy controls(139.81±23.35pg/ml) (all P<0.05). There were increased significantly in static and paracmasis compered to progressive of PV (all P<0.05,). The expression of IL-38 in static were higher than in paracmasis, but there was no significants (P>0.05).Conclusion:IL-33 and IL-38 may play a major role in the pathogenesis of psoriasis vulgaris.Part2Expression of IL-33,IL-38 in peripheral blood of patients with Henoch-Schonlein purpura.Objective:To detect the expression of IL-33, IL-38 in peripheral blood of patients with Henoch-Schonlein purpura.Methods:Peripheral blood was collected from patients with Henoch-Schonlein purpura in acute stage (n=29) and convalescent stage (n=27) as well as 30 healthy human controls.A competitive enzymelinked immunosorbent assay(ELISA) was performed to determine the levels of IL-33 and IL-38.Results:The serum IL-33 levels were significantly higher in the patients with acute and convalescent stage than in the healthy human controls ((90.78±18.45pg/ml), (74.54±11.78pg/ml) and (58.14±16.22 pg/ml), respectively). The serum IL-33 levels in acute stage were significantly higher than those in convalescent stage (t=3.891,p (0.01). The serum IL-33 levels in convalescent stage were significantly higher than those in the healthy controls (t=4.326,P (0.01). The serum IL-38 levels were significantly lower in the patients with acute and convalescent stage than in the healthy controls ((34.02±12.07pg/ml), (83.49±14.98 pg/ml) and (130.58+ 27.08pg/ml), respectively). The serum IL-38 levels in acute stage were significantly lower than those in convalescent stage (t=13.654, P (0.01). The serum IL-38 levels in convalescent stage were significantly lower than those in the healthy controls (t=7.995, P (0.01).Generally,the serum levels of IL-33 in HSP patients were positively correlated with IL-38(r=0.522,P< 0.01).Conclusion:IL-33 and IL-38 may play a major role in the pathogenesis of Henoch-Schonlein purpura and serve as potential indicators for estimation of this disease.