A Proteomic Study Of Brain Tissue And Plasma In Focal Cerebral Ischmic Rats And The Protective Effects Of Isoteviol

Ischemic stroke is the third leading cause of mortality. The pathological changes of signal transduction pathways are not clearly understood. In this study, using high throughput shotgun proteomic method based on LC-MS/MS to determine the proteins in brain tissue and plasma of cerebral ischemic rats, identifying and analyzing proteins associated with different pathways.In addition, STVNa was verified to have brain protective effects, we also study the role of STVNa on regulating above-mentioned pathways. Following is the main content of this study:Sprague-Dawley rats were divided into three groups, i.e. the control group, the ischemic group(Middle Cerebral Arteria Occlusion, MCAO), and the treatment group(MCAO and 10mg/kg STVNa). Brain tissue cytoplasma and plasma were collected, fractionated by strong cation exchange, and identified by LC-MS/MS.1) Mass Spectrometry data indicates that 176 and 200 specifically expressed plasma proteins were identified in control and ischemic group. Six of which proteins were brain-specific proteins determined by search of gene expression tissue-specificity.2) Mass Spectrometry data indicates that 648 and 147 specifically expressed brain proteins were identified in control and ischemic group. Pathway analysis shows that many pathways associated with inflammation, apoptosis, self-repair were found at 4h after cerebral ischemia.3) Mass Spectrometry data indicates that 279 specifically expressed brain proteins were identified in treatment group. Pathway analysis shows that STVNa could inhibit the enhancement of cell growth pathways and the reduction of inflammation pathways after ischemia, while promoting carbohydrate metabolism and fatty acid metabolism associated pathways.Conclusions: 1) Identification of the brain-specific specifically expressed proteins in ischemic plasma can provide candidate proteins for the research on biomarker of stroke. 2) Pathways of brain tissue associated with inflammation, cell death, self-repair are relatively active after 4h cerebral ischemia. 3) STVNa can inhibit inflammation, promote cell growth, improve metabolic remodeling, and protect cerebral ischmia.