Effects Of Bone Marrow Mesenchymal Stem Cell Transplantation On Expression Of Transforming Growth Factor-β1and Tumor Necrosis Factor-α Level Of Emphysema Rats

Objective:By research the expression of TGF-β1 and TNF-α level in lung tissue and serum after transplantation of bone marrow mesenchymal stem cells(BMSCs) to emphysema rats,study the effects of BMSCs on airway inflammation and remodeling of emphysema rats.Methods:36 healthy male SD rats were selected randomly and they were divided into control group, simple emphysema and BMSCs transplantation group. Eemphysema rat models were established by exposure to chronic tobacco smoke with reference to Xu Sanlin chronic smoke exposure device, six months later,the BMSCs suspension was infused into the tail vein of BMSCs translation group rats,and the equal amount of PBS buffer was infused into the control group.The relevant indices were measured after BMSCs transplantation(15 days later),the pathology changes of lung tissue were observed and the mean linear intercept(MLI)、mean alveoli number(MAN) and total area of the tube wall((WAt)/wall inside the perimeter(Pi) were calculated after HE staining;the levels of TGF-β1 and TNF-α in lung tissue and serumwere detected by ELISA kits;the TGF-β1expression of airway epithelial cells was measured by immunohistochemical method.Results:1. The microscopic morphology change: pure emphysema and BMSCs transplantation group both have different degrees of emphysema pathological changes, the latter isIV significantly lighter than the former.2. The MLI(177±22.14)um and WAt/Pi(193.05±53.91)um in the simple emphysema group were obviously higher than that of control group[(95±44.28)um and(78.39±26.51)um, all P < 0.05); The TNF-α[(14.47± 5.85) and(92.07± 23.73)] ng/l and TGF-β1[(66.77± 9.91) and(72.92 ±4.96)] ug/l levels in lung tissue and serum, the TGF –β1average optical density value of the positive particle in airway epithelial cell(6.44±2.40)×10-2of the simple emphysema group were obviously higher than that of control group[(5.17 ± 2.57)and(53.49± 15.47) ng/l、(49.02± 9.91)and(59.36± 6.89) ug/l and(3.83 ±1.81)×10-2, all P <0.05];the MAN(92.73±28.93) /mm2 was obviously lower than that of control group [(190.84±57.49/mm2, P<0.05)]; The MLI( 131±37.55) um and WAt/Pi(121.62±34.94) um in the BMSCs transplantation group were obviously higher than that of control group; The TNF-α[(9.60±5.06)and(70.92± 15.16)]ng/l 、TGF-β1[(57.44± 4.78)and(66.43 ± 6.70)] ug/l levels in lung tissue and serum and the TGF–β1 average optical density value of the positive particle in airway epithelial cell( 4.28±1.23)×10-2in the BMSCs transplantation group were obviously higher than tha control group(P<0.05), the MAN(146.73±49.12) /mm2 was obviously lower than that of control group(P<0.05); while the BMSCs group were obviously lower than simple emphysema(P < 0.05).3. In each group, the lung tissue and serum TGF-β1 level correlated positively with TNF-α level(r=0.556, P=0.001)and WAT(r=0.702, P<0.01; The lung tissue and serum TNF-α level correlated positively with MLI(r=0.578,P=0.001)and WAT(r=0.440,P=0.015), and correlated negatively with MAN(r=-0.439,P=0.015); WAT was positively correlated with MLI(r=0.398, P=0.029), and negatively correlated with MAN(r=-0.475,P=0.008).Conclusions:Transplantation of BMSCs can reduce the TGF-β1 and TNF-α levels in lung tissue and serum of emphysema rat,then reduce airway inflammation and remodeling, and thus improve the severity of emphysema.