Change Of IL-27、RANKL In Patients With Rheumatoid Arthritis And Its Influence On Osteoporosis

Objective:Through testing serum levels of interleukin(IL- 27) and nuclear factor kappa B receptor activation factors predominate the change of the ligand(RANKL) of patients with rheumatoid arthritis(RA) and normal group, research the relationships with disease activity, and preliminary discussion their effects on osteoporosis in patients with RA.Methods:The first hospital of shanxi medical university for the first time of 55 patients with diagnosed with RA, consistent with 2010 ACR/EULAR classification criteria of RA. Patients without using any immunosuppressant and taking hormone, there is no history of anticoagulant and affect bone metabolism of drugs, without severe renal damage, thyroid, parathyroid disease. 42 patients age and gender matched normal persons as control group, its early in the morning on an empty stomach blood collection, save the serum and separation. Were measured by ELISA method in 55 patients with RA and 42 cases of normal serum IL- 27 and RANK L levels, Use the calcaneus ultrasonic bone mineral instrument measurement test bone mineral density. And then analysis RA patients serum IL- 27, RANKL levels and both its relationship with disease activity index, and analyzes the relationship with osteoporosis. Results:1. The RA group of bone mineral density significantly lower than normal group(P = 0.004), and the RA group, the incidence of osteoporosis was 52.8%, significantly higher than the control group 19.1%;2. RA serum IL- 27, RANKL and IL- 27 / RANKL ratio, IL- 27 with the control, RANK and IL- 27 / RANKL ratio than were significantly increased(P < 0.05);3. The RA group, IL- 27, RANKL, IL- 27 / RANKL and reaction disease activity index number of joint swelling, joint tenderness, white blood cell count, hemoglobin, blood platelets, CRP, ESR, anti CCP antibody had no correlation(P > 0.05);4. in RA group, levels of IL – 27、 RANKL、 IL- 27 / RANKL have no correlation with BMD, and normal bone mass, bone loss, osteoporosis in the three groups of IL- 27, RANKL, IL- 27 / RANKL ratio had no difference(P > 0.05). Conclusions:1. Rheumatoid arthritis, bone mineral density is reduced, and there is a higher incidence of osteoporosis;2. In patients with RA,serum level of IL- 27, RANKL level and IL- 27 / RANK L ratio were significantly elevated, but have no relationship with disease activity index,suggesting that IL-27、RANKL can not reflect disease activity; 3. IL- 27 may affect bone metabolism of patient with RA through a certain mechanism that needs further study.