The Relationship Between Heme Oxygenase-1 Gene Polymorphisms And Non-culprit Lesions After Percutaneous Coronary Intervention In Coronary Heart Disease Patients

Objective: To research the relationship between the polymorphism of heme oxygenase-1(HO-1) gene promoter GT repeat sequence and non-culprit lesions after percutaneous coronary intervention(PCI) in coronary heart disease patients.Methods: The coronary artery disease patients with the first time of PCI surgery were followed up, observing the situation of non-culprit lesions after six months, a total of 282 cases. The subjects were divided into progressive group and non-progressive group. Peripheral blood of the patients was extracted.DNA was abstracted and the HO-1 gene promoter GT repeat sequence were amplified PCR. The length of GT repeat fragment was identified by polyacrylamide gel electrophoresis.Hardy-Weinberg balancing applications will ensure that the selected samples have a group representation. By application of X2 test,comparing two groups of allele frequencies to identify related polymorphism. The different groups’ OR values(odds ratio) were calculated to analysis of relative risk.Compared the relationship between the polymorphism of heme oxygenase-1(HO-1) gene promoter GT repeat sequence and non-culprit lesions after stent Implantation in coronary heart disease patients.Results: Compared with non-progressive groups, the patients in progressive group had less rate of carrying S alleles(X2=10.326,P<0.05).By multivariate logistic analysis, the progression of the non-culprit lesions in patients was related to the HO-1 gene polymorphism, LDL, and other factors. The patient carrying S alleles was a protective factor to progress of non-culprit lesions(OR=0.483,95%CI 0.263=0.885, P<0.05).Conclusion: The HO-1 gene promoter GT repeat sequence was related to the progression of non-culprit lesions in coronary heart disease patients after stent Implantation. The patients with S gene had less relations with the progression of non-culprit lesions.