Eucheuma Gelatinae Polysaccharides(EGP) Extraction Technics Optimization And EGP Biological Activity Evaluation

We aimed to optimize the ultrasonic extraction process of Eucheuma gelatinae polysaccharides(EGP). Extraction conditions including the extraction time, temperature as well as the material to solvent ratio were optimized by orthogonal experimental design(OED).The optimum extraction condition and yield were confirmed. The obtained EGP were used for the following experiments. 1. Identification tests including: dissolution experiment, ninhydrin reaction, Iodine-potassium iodide reaction and Infrared spectroscopy experiment. 2. The determination of nucleic acid and protein concentrations using UV-Spectrophotometric method 3. the content of polysaccharide was measured using phenol-sulfuric acid method. 4. To investigate its biological activity and the applications of EGP in the cosmetics industry. The EGPs repair and protection activities were evaluated in terms of cell morphology, cytotoxic activities, cell growth and cell migration on Ha Ca T cell line. To study the function of EGP in inflammation, we established the LPS-induced inflammation model. We used flow cytometry and ELISA experimental to detect the released ROS and NO in Ha Ca T cells. It adopted the physical method to evaluate the EGP’s moisture retention and the hygroscopicity. We also discussed the value of EGP from the aspect of UV shielding and absorbing in cosmetics industry. The anticancer properties were evaluated in vivo using He La cells、Si Ha cells、BT474 cells、Ca Si Ka cells and 9706 cells.The optimized extraction process was at the following conditions: 60℃, solid-liquid ratio of 1:100, 30 min, and EGP yield 40.3 % ± 1.8 % and with the polysaccharide content of 48.2 % ± 4.6 %. EGP’s dissolution experiment, ninhydrin reaction, Iodine-potassium iodide reaction and Infrared spectroscopy experiment were all positive results which showed the extract samples contained polysaccharide substances. Cell proliferation and migration assays demonstrated that EGP played an important role to facilitate the Ha Ca T cell proliferation and migration with no toxicity. The content of promoting cell growth factor hydroxyproline(Hyp) in the EGP group was 145.09±16.7 μg/L at 24 h and it was higher than that in the Normal group(110.72±8.7 μg/L) and b FGF(110.72±8.7 μg/L) group. The content of promoting cell growth factor-I type collagen(COL-I)(68.81±4.3 μg/L) at 0 h in EGP group and it was significantly higher than that in the Normal Group(21.08±2.1 μg/L) and b FGF(27.91±1 μg/L) group. That might accelerate shin repairment and wound healing. In the inflammation modle, EGP had certain inhibitory effect in i NOS inflammatory factor. EGP showed a significantion reduce in ROS and nitric oxide in oxidative stress experiment. EGP presented the inhibition effects in UV absorption and shielding with concentration dependence, and distinct moisture absorption capacity but no hydrating quality. The moisture absorption rate of EGP can reach 11.69% in 80% humidity. Evidence from various activities studies suggested EGP have not great potential for possible applications in cancer prevention.Conclusion: It was an energy saving and stable technology to extract EGP by ultrasound. The obtained EGP could improve the wound healing and regeneration of skin and mucosa membrane with no toxicity. EGP showed greated potential in the fields of cosmertics and therapeutic devices.