EGFR Expression In Ovarian Epithelial Tumors And RNAi Targeted Action Research

Objective:1.The Epidermal growth factor receptor(Epidermal growth factor receptor,EGFR) expression in ovarian epithelial tumors.2. Explore the expression of EGFR gene and RNA interference on the impact of tumor cell proliferation.Methods:1.Select 20 cases of ovarian benign tumor(benign group), 40 cases(border)border oarian tumors, ovarian epithelial carcinoma 40 cases(malignant group). Using immunohistochemical detection of the expression of EGFR protein. Statistical methods to analyze each group EGFR gene expression.2.Build si RNA targeting EGFR gene interference expression vector, transfection into SKOV3 cells. The experiment is divided into three groups: control group, blank plasmid transfection group, specificity transfection group. Rt-pcr detection of EGFR gene expression. Determined by MTT method analysis of cell proliferation. Tablet colony inhibition experiment calculation.Results:1.Border ovarian benign tumors, ovarian tumors, ovarian epithelial carcinoma in EGFR positive expression rate were 20.0%, 47.5%, 85.0%, the positive expression rate of malignant group is higher than the other two groups(P < 0.05), benign group compared with border group does not have statistical significance(P > 0.05). Positive expression rate of EGFR and clinical stage, pathological classification and related to the size of the tumor(P < 0.05), has nothing to do with the age of the patients(P >0.05).2.Successful build si RNA targeting EGFR gene interference expression vector,transfection SKOV3 cells, rt-pcr analysis showed that specific transfection group and the blank cells, EGFR m RNA expression plasmid group was obviously suppressed.The logarithmic phase of different groups of cells count, determined by MTT cell growth curve drawing, found that the differences between different groups of cellproliferation, cell growth specificity transfection group was obviously lower blank plasmid group and the control group, cell inhibition rate was 61.8%. Tablet colony experiment, specific group transfection cell colony formation inhibition rate was59.9%.Conclution:1.Border EGFR protein in ovarian tumors and ovarian epithelial carcinoma has expressed, and gradually increased. Prompt EGFR gene may be a important factor in the development of ovarian epithelial tumor formation.2.Targeting the EGFR gene RNA interference can reduce the expression of EGFR, SKOV3 cells leads to cell death, reducing cell proliferation. So the targeting EGFR RNA interference technology used in ovarian epithelial tumor target gene therapy research has certain feasibility.