The Basic Research Of Yak Activated Protein On Anti-radiation Injury

Objective: Ionizing radiation plays a positive role in agricultural production, medicine and health, scientific research, and national defense. However, the damage it causes, including to the peripheral blood cells, bone marrow DNA, immune-related organs, and antioxidant enzymes, in a growing number of workers cannot be ignored. Therefore, identifying an effective natural medicine that protects against radiation and reduces the related damage is an urgent problem in the field of radiation medicine. The aim of this study was to test amino acid content of Yak-activated protein, murine immunoregulation, anti hypoxia, anti fatigue and decrease blood glucose effect. Analyze the effect of Yak-activated protein on peripheral blood cells, immune function, bone marrow DNA content, antioxidant enzyme activity, and the expression of apoptosis-related proteins expression in radiation-induced injury in mice. We also explored the mechanism behind the possible protective effects of Yak-activated protein, and provided novel ideas and methods for studying radiation protective agents. Methods: Evans Blue colorimetric, MTT colorimetric method, semi-vivo method and modified Jernes method were adopted to determine the delayed-type hyper-sensitivity induced by dinitrochlorobenzene, lymphocyte proliferation, and the ability of phagocytosis and the title of hemolysin antibody, respectively. To evaluate the immune regulation of Yak-activated protein. The anti hypoxia, anti fatigue and hypoglycemic effect of Yak-activated protein were evaluated by the determination of the survival time, the swimming time and the blood glucose of mice, respectively. A total of 180 Kunming mice were divided randomly into three groups: A, B, and C. Sixty were divided randomly into the normal control group, a radiation model group, a positive control group, and high, medium, and low Yak-activated protein dose groups(10, 5, and 2.5 mg/kg, respectively). Yak-activated protein was administered orally in the treatment groups, whereas an equal volume of normal saline was applied orally to the normal control and radiation model groups for 14 d. The positive control group received amifostine(150 mg/kg) via intraperitoneal injection. All mice apart from those in the normal control group received a whole-body X-ray once 3 d, 7 d, and 14 d after irradiation. The changes in the peripheral hemogram, thymus and spleen indices, Colorimetric determination method DNA content in the bone marrow, SOD、GSH-PX、CAT、T-AOC antioxidant activity and MDA content. Elisa method was used to test IL-2 and IL-6 levels, and the expression of Bcl-2 and Bax protein after irradiation were then assessed. Results:Yak-activated protein has a good effect on murine immunoregulation Yak-activated protein had obvious effects on organ indices. The hemogram results increased gradually in the low-Yak-activated protein dose group, the DNA content in the bone marrow was increased in the yak-activated protein groups. The SOD, GSH-PX, CAT, T-AOC, antioxidant enzyme activity were enhanced and the content of MDA reduced in the yak-activated protein groups. The IL-2 content was increased significantly in the yak-activated protein groups. In addition, the expression of Bcl-2 and Bax was increased and decreased, respectively. Conclusion:Cell damage can effectively inhibit the activity of Yak-activated protein induced by radiation, the peripheral hemogram gradually restored, can enhance the body’s antioxidant enzyme activities better, reduce lipid peroxide in vivo stack, the protection of bone marrow DNA and immune organs from damage, improve immunity, can effectively regulate the secretion of cytokines and expression, inhibiting cell apoptosis radiation protection. Yak-activated protein exerted protective effects on X-ray radiation-induced injury in mice.