MiR-155-5p,miR-21-5p And MiR-29a As A Biomarker For Diagnosis Of Tuberculosis

Objective This paper aims to investigate expression levels of mi R-155-5p, mi R-21-5p and mi R-29 a in tuberculosis patients infected, latent tuberculosis and healthy controls, to explore its potential as a biomarker for diagnosis of tuberculosis.Methods Selected subjects from the hospital, every capita were make QFT(QuantiFERON- TB Gold In-Tube, QFT) diagnosis, then select the subjects from the test results. We collected 20 patients with pulmonary tuberculosis from hospital patients, and choose 20 cases of latent tuberculosis infection form TB patients families and physical examination. 20 cases of healthy controls selected from a physical examination. Heparin tubes were used to take 5ml peripheral venous blood of each subjects, and processed blood within 4 hours, plasma was separated and was extracted total s RNA(small RNA) from plasma,disposed s RNA with poly(A), and finally, using Oligo(d T)-Universal Tag conduct s RNA reverse transcriptase. The expression level of mi R-155-5p, mi R-21-5p and mi R-29 a are detected by reverse transcription- quantitative PCR. Statistical analysis of measurement data using mean ± SD, ANOVA comparing mi RNA differences between multiple groups, P <0.05 was considered statistically significant.Results(1) Latent tuberculosis infection rate was 43.8% in people who is a family of patients with active pulmonary tuberculosis, the rate in healthy subjects was 21%.(2) The expression of mi R-155-5p,mi R-21-5p and mi R-29 a were upregulation in active pulmonary tuberculosis patient’s plasma for 12.27 times, 4.73 times and 3.81 times compared with healthy controls. the difference was statistically significant(p <0.05 for all); ROC analysis of active pulmonary tuberculosis patient of mi R-155-5p, mi R-21-5p and mi R-29 a were 0.938, 0.880, 0.808 compared with health to controls.(3) Compared with latent tuberculosis infection, the expression of mi R-155-5p, mi R-21-5p and mi R-29 a were upregulate for 3.82 times, 1.98 times and 8.50 times in active tuberculosis patient, statistics analysis showed that mi R-29 a was significantly(p <0.05).(4) Latent TB infection of mi R-155-5p, mi R-21-5p were upregulation for 3.21 and2.39 times compared with healthy controls, the difference was statistically significant of mi R-155-5p(p<0.05), no statistically significant differences in expression of the mi R-29 a tested could be identified; ROC analysis of mi R-155-5p with the area under the curve(AUC) was 0.845.Conclusion The expression levels of mi R-155-5p, miR-21-5p and miR-29 a were differences in healthy controls and active tuberculosis, the expression levels of mi R-155-5p、mi R-29 a were differences in latent tuberculosis infection and active tuberculosis, the expression levels of mi R-155-5p were differences in latent tuberculosis infection and healthy controls. Speculated that mi R-155-5p, mi R-21-5p, mi R-29 a have differentially expressed in different patients, there is a certain relationship with the occurrence and development in tuberculosis which may be a potential diagnosis biomarkers to distinguish healthy controls,active tuberculosis and latent tuberculosis infection. Latent tuberculosis infection rate was higher in people who is a family of patients with active pulmonary tuberculosis than healthy subjects.