Inhibitory Effect Of Novel High Polymer Antibacterial Endotracheal Tube (NHPAET) On Pseudomonas Aeruginosa Biofilm Formation In Vitro

Objectives To study the different inhibitory effect on Pseudomonas aeruginosa biofilm formation in vitro among NHPAET-1 and erythromycin-coated endotracheal tube and common endotracheal tube in order to provide the relative research data for clinical application.Methods Completely random design was employed. The biofilm models of Pseudomonas aeruginosa in vitro were made for NHPAET-1 and erythromycin-coated endotracheal tubes and common endotracheal tubes, then the viable bacterial counts within biofilm were determined and the biofilm was examined by scanning electron microscopy (SEM) or silver staining on 3rd day and 7th day.Results1. Dissolution test of antibacterial materials: inhibition zone width (D) is zero and NHPAET-1 is no dissolution material.2. Antibacterial property test: the antibacterial rate of NHPAET-1 is over 99% and antibacterial ability is powerful, based on the evaluating criteria.3. Test of viable bacterial counts within biofilm: the bacterial survival in common endotracheal tube group was significantly improved compared with NHPAET-1 group (P<0.001), while a statistically significant difference was detected between common endotracheal tube group and erythromycin-coated endotracheal tube (P<0.01).4. Inhibitory effect of NHPAET-1 on biofilm information: biofilm can be found on common endotracheal tube surface, but there is no biofilm on NHPAET-1, by means of SEM or silver staining on 3rd day and 7th day. Furthermore, the 3rd day biofilm information is less than 7th day on erythromycin-coated endotracheal tube surface by SEM.Conclusions NHPAET-1 is the no dissolution material and has powerful antibacterial ability, meanwhile, NHPAET-1 can resist efficiently Pseudomonas aeruginosa biofilm formation in vitro.