Quercetin Enhanced The Chemotherapentic Effect Of Adriamycinin Leukemia And To Reduced Myocardial Toxicity Experiment

Objective We use the quercetin in P388 leukemia to transplante tumor nude mouse, for studying the effect of anti-leukemia, enhancing the chemotherapeutic and extenuating adriamycin myocardial injury and its mechanism in vivo. Methods We use the logarithmic growth phase of P388 leukemia cells into Balb/c nude mouse subcutaneously for building leukemia mouse model,and therapy leukemia mouse with quercetin, adriamycin and this two combination to observe the changes of the survival time of mouse.In this time,we observe the effect of quercetin on cell proliferation by flow cytometry cell cycle assay; detect Caspase-3 protein levels with ELISA method to explore the mechanism; detect the changes of NF-κB, Bcl-2, Bax gene and protein by real-time fluorescent quantitative PCR and Western-blot; detect the changes of cardiomyocytes in mice by electron microscope; detect the activity of oxygen free radicals related enzymes to explore its protective effect on oxidative damage of myocardial cells. Results Quercetin and adriamycin could significantly prolong the survival of P388 leukemia in nude mouse, and the two compared with the single drug group are better; quercetin and doxorubicin alone or in combination can reduce tumor tissue of mouse leukemia cells ratio of G0-G1 phase, S phase and G2-M increased and combination could be better; quercetin could activate Caspase-3 enzyme and promote leukemia cells apoptosis, at the same time, it could up-regulate Bax and down-regulate Bcl-2 and NF-κB. Transmission electron microscope results show, quercetin can obviously improve the damage effect of adriamycin on cardiac; quercetin could protect myocardial injury by oxygen free radicals.Conclusion Quercetin could significantly prolong the survival time of P388 leukemia nude mouse; quercetin can inhibit P388 leukemia nude mouse leukemia cell proliferation in S phase; quercetin can promote apoptosis; quercetin can anthracene the adriamycin doxorubicin and the two combined are significantly increased; quercetin could protect myocardial injury by its antioxidant effects,and it provides a theoretical basis for further research and development.