The Protective Effects Of STVNa On Vascular Remodeling In Pulmonary Arterial Hypertension In TAC Rats

Pulmonary arterial hypertension due to transverse aortic constriction(PAH-TAC) is a common pulmonary arterial hypertension in clinic. There is no specific treatment for the therapy of this disease. Now it is considered the mechanism of PAH-TAC relates to ROS overproduction, thus leading to vascular smooth muscle cell proliferation in resistance artery. Our research focus on the vascular remodeling therapeutical effect and the potential mechanism of STVNa on PAH-TAC. The main contents are as follows:Adult male Wistar rats(n=3) were performed transverse aortic constriction surgery. After 9 weeks, left ventricular end diastolic pressure, femoral artery pressure, cardiac output, heart rate, were chronologically collected. The results showed that compared with the sham operation group, the left ventricular end diastolic pressure increased obviously, while the femoral artery pressure, cardiac output, heart rate decreased significantly in model group. The paraffin sections of lung tissues were stained by hematoxylin-eosin staining. The results show that a marked medial muscularization and reduced pulmonary artery lumen size occur in the model group. In addition, compared with model group, STVNa administration group and Sildenafil positive control group decreased significantly in pulmonary arterial vascular medial thickening and vascular muscularization. What’s more, there is a dose-relevant manner in STVNa administration groups. Compared with 70 mg/kg Sildenafil, the effect of 4 mg/kg STVNa is better in reversing vascular remodeling. Our research also observed the effect of STVNa on NO and ROS of vascular smooth muscle cell and bronchial smooth muscle cell by laser sanning confocal microscope and preliminary investigate the mechanism of STVNa on PAH-TAC. In conclusion,(1) Our research established a stable PAH Rat model.(2) Our research shows that STVNa alleviates PAH by inhibiting vascular medial thickening and vascular muscularization.(3) Compared with experimental drugs sildenafil(PDE5 inhibitor) in clinic, the effects of STVNa is more obviously in vascular remodeling. ROS production can not be increased by STVNa. The potential mechnism of STVNa may be involed in regulation of c GMP and ROS...