Combination Of IL-24 And Cisplatin Inhibits Lymph Node Metastasis Of Uterine Cervical Cancer Xenografts

Objectives:To investigate the inhibitory effect of p DC316-h IL-24 combined with cisplatin on lymph node metastasis in nude mice with human cervical cancer. Methods:Nude mice cervical cancer transplant tumor models were established and randomly grouped into 5 treatment schedules:A:PBS;B:PDC316 vector; C:p DC316-h IL-24; D:cisplatin(5 mg/kg) and E:p DC316-h IL-24 combined with cisplatin. Observed and record the nude mice general condition, the tumor growth and the Lymph node metastasis; The expression of m RNA of IL-24 in transplanted tumors was identified by PCR;The tissue is the metastasis lymph node confirmed by immunohistochemistry. Expression of(Caveolin-1)and VEGF-D in lymph node was respectively detected via polymerise chain reaction and western blot. The effect of IL-24 gene combined with cisplatin on human cervical lymph node metastasis of Siha cells was evaluated by comparing the expression of Caveolin(Caveolin-1) and VEGF-D and the metastasis number of lymph node between the groups. Results:Gene recombinant plasmid p DC316-h IL-24 was successfully transfected and expressed. The tissue is the metastasis Lymph node. After interfere, The lymph node metastasis of combined group was the lowest(1.57±0.79), the difference was statistically significant(P<0.05); the combined group lowered the expression of VEGF-D(VEGF-D was 0.12±0.00) and raised the expression of Caveolin-1(Caveolin-1 was 0.74 ± 0.01) in the most obvious effect. Conclusions:1.The inhibiting effect of IL-24 gene in combination with cisplatin on lymph node metastasis is superior to cisplatin alone through down-regulating the expression of VEGF-D and up-regulating the expression of Caveolin-1;2. Caveolin-1 and VEGF-D are interrelated in the lymph node metastasis in cervical cancer.