Research On Inhibition To Human Uterine Lymph Node Metastasis Through MTOR/P70S6K Signaling Pathway With IL-24 And Cisplatin

Objective:TO investigate m TOR/P70S6 K signal pathways are involved in cervical cancer of siha cell of lymphatic metastasis, and p DC316 Hil-24 plasmid gene combined cisplatin in the way to play a role of inhibition.Methods:Nude mice xenograft models were established with human cervical cancer Siha cells,and then animal models were randomly divided into 6 groups with 7 mice in each group: PBS, IL-24, low-dose DDP, high-dose DDP, IL-24 combined low dose DDP and IL-24 combined high-dose DDP, After the intervention, through observe the conditions of weights and estimate the lymph node metastasis of our mouse, using CK keratin was tested to whether lymphoid tissue was transplanted tumor lymphatic metastasis, In addition,there was a detection that the expression of m RNA of IL-24 in lymphoid tissue by PCR technique, Finally, the expression of m TOR and P70S6 K were tested by immunofluorescence technique as well as western blot imprinting method to quantitative analysis of its expression.Results:①There are IL-24 in nude mice xenograft lymphoid tissues, Showed that it was successful transfection ②The CK keratin for each group was successfully tested in carcinoma infiltrating lymphoid tissues, ③After intervention, the numbers of Metastasis lymph node in different groups were different, that is, the combined group was obvious lower than others(p<0.05),moreover, there was no statistically significant difference(p>0.05) on IL-24 + low-dose cisplatin group and IL-24 + high-dose cisplatin group ④m TOR/ P70S6 K was successfully tested in all of groups by Immunofluorescence technique,and Quantified by Western blot analysis, comparison with other groups, the combined group was significantly decreased(p<0.05), the difference was statistically significant, and the expression of each two proteins has significant positive correlation(r = 0.968,p<0.001).Conclusions:It is concluded that IL-24 combined with DDP in body inhibit the activity of m TOR /P70S6 K signaling pathway to promotes cell apoptosis signaling, from which human cervical cancer cells would be inhibited in nude mice growth and lymph node metastasis.The therapeutic effect on IL-24 + low-dose DDP group is same with IL-24+high-dose DDP, moreover, IL-24 + low-does DDP can significantly inhibits tumor growth and lymph number of metastasis.