The Effects Of Matrix Metalloproteinase Inhibitors Of GM6001 、MMP2/9inhibitorⅠand Mmp2/9inhibitorⅡon Migration Of Human Lens Epithelial Cells

Objective:To determine whether metalloproteinase inhibitor can prevent the migration of human lens epithelial cells in search for the basis for clinical medication to prevent PCO following cataract surgery.Methods:LECs were cultured in vitro. The second or third generation of cells were incubated to6-well plates. Then the cells were cultured in Dulbecco’s modified Eagle medium(DMEM)for 12 hours, which didn,t contain fetal bovine serum when the cells presented a 70%confluent monolayer. GM6001、MMP2/9InhibitorⅠand MMP2/9InhibitorⅡ were added into the culture medium with different concentrations(0.25, 0.5, 1, 2, 4, 8, 16, 32, 128μmol/L)for 24 hours separately, and regular cultured cells served as the control group. A bare area was made by a 200μl sterile spearin the cell layer. The migration distance and inhibition ratio were calculated after 24 hours. The second or third generation of cells were incubated to 96-well plates at the density of 5×105/ml(200μl/well) for 24 hours. GM6001(128μmol/L)、MMP2/9InhibitorⅠ(64μmol/L) and MMP2/9InhibitorⅡ(32μmol/L) were added into the culture medium for 24 hours and the cell viability was tested by using MTT assay.Results:(1) Cultured cells grew well with irregular arrangement and presented thepolygon in shape.(2) Epithelial cells migration distance was significantly reduced by GM6001 、MMP2/9InhibitorⅠand MMP2/9InhibitorⅡ in a dose dependent manner as compared with the control group, the rank correlation coefficients were 0.847、0.856 and 0.901 and they were all higher than 0.5.(3) Cell migration distance of MMP2/9InhibitorⅡgroup was obviously lower than the other three groups: The cell migration distance of the control group was set to 1, and the migration distances were 0.478±0.091 、 0.294±0.088 and0.191±0.081, GM6001 group(32μmol/L) 、 MMP2/9InhibitorⅠgroup(32μmol/L) and MMP2/9InhibitorⅡgroup(32μmol/L), respectively, showing a significant difference among the groups(F=116.031, P<0.01).(4) The OD values were 0.607±0.0163 、0.567±0.0151 、 0.583±0.0103 and 0.595±0.0138 in the control group, GM6001 group(128μmol/L) 、 MMP2/9 InhibitorⅠ group(64μmol/L)and MMP2/9InhibitorⅡgroup,respectively, with no significant difference among the groups(F=1.403, P>0.05).Conclusions:The three inhibitors inhibit the lens epithelial cells mobility and do not affect the cell viability in cultured lens epithelial cells in vitro. MMP2/9InhibitorⅡ has the most obvious inhibitory effect between the three inhibitors and it may has a role in the treatment of posterior capsule opacification.