Detection Of EB Virus DNA In Patients With Acute Leukemia And Its Clinical Significance

Objective To investigate Epstein Barr virus(EBV) infection in the patients with acute leukemia(AL), explore the relevance to the generation, progress and prognosis of AL,discuss the association between EBV and chromosomal changes.Methods Nested polymerase chain reaction(PCR) was used to detect EBV in 50 cases of acute myeloblastic leukemia(AML), 34 cases of acute lymphocytic leukemia(ALL)and 20 cases of healthy control group. Karyotyping was performed in AL patients by Rbanding.Results The EBV positive rate of ALL, AML patients and healthy controls was 58.8%(20/34), 34%(17/50) and 10%(2/20), respectively. There were difference on the overall EBV positive rate among the three groups(χ2=13.3, P<0.05).The comparison between AML group and ALL group 、 healthy control group in the infection of EBV had no significant difference(χ2=5.1, P>0.0167; χ2=4.2, P>0.0167). The positive rate of EBV in ALL was higher than that of the healthy group(χ2=12.4, P<0.0167). Chromosome karyotype analysis showed that 64.7%(22/34) of patients with ALL and 40%(20/50) of patients with AML were detected abnormal karyotype. The rate of abnormal karyotype in ALL group was higher than that of AML(χ2=4.94, P<0.05). Chromosomal abnormalities were detected in 23 of 37(62.2%) patients with EBV positive, while 19 of the 47(40.4%)patients with EBV negative. The chromosome abnormalities of patients with EBV positive was higher than that of EBV negative(χ2=3.9, P<0.05)Conclusion EBV infection may relate to chromosome changes of AL and onset of patients with ALL.