Analysis Of Mirnas Expression Profile In Plasma On Different Subtypes Of Ischemic Stroke

ObjectiveTo investigate the circulating mi RNAs expression profile changes in different subtypes of ischemic stroke according to TOAST.Methods1. We selected 16 patients who were diagnosed as acute ischemic stroke at first time in the department of neurology, the Affiliated Hospital of Qingdao University from November to December in 2012, they were divided into large artery atherosclerosis(LAA) stroke group including 8 cases, small artery occlusive(SAO) stroke group containing 8 cases, and 8 health checkup patients with the same period were selected as control. High-throughput sequencing was used to detect the expression profiles of mi RNAs in the plasma.2. The mi R-146b-5p, mi R-23a-3p and mi R-451 a of high-throughput sequencing results were validated by quantitative real-time polymerase chain reaction.3. We performed the mi RNAs variance analysis and associated bioinformatics analysis.ResultsThirty hundred and sixty-nine mi RNAs were detected in LAA group, SAO group as well as the control group. We found remarkable differences(fold change>2 and P<0.01) in the expression of 12 mi RNAs including let-7a-5p,mi R-744-5p etc. between any two groups of the three groups. And 34 mi RNAs containing mi R-126-5p, mi R-23a-3p,mi R-143-3p etc. had a lower expression(fold change>2 and P<0.01)in LAA group than that in the control group. In comparison with the control group, mi R-1304-3p,mi R-451 a were significantly down-regulated(fold change >2 and P< 0.01), while 27 mi RNAs were found significantly up-regulated(fold change >2 and P<0.01) in SAO group.1. The expression levels of mi R-146b-5p, mi R-23a-3p, mi R-451 a were validated in accordance with the results of real-time PCR(q RT-PCR).2. Target gene prediction and functional analysis reveal target genes regulated by differential expression of mi RNAs are mainly associated with cell proliferation,adhesion, metabolism and other biological functions.Conclusionmi RNAs express differently in the plasma of LAA group, SAO group and healthy control group, which reveals that mi RNAs may play their different roles in the pathogenesis of LAA stroke and SAO stroke by regulating their downstream target genes.