The Role Of IFN-γ Regulating CIK Effects T Cells Sub-population And The Evaluation Of Antitumor Cell Activity

Objective: To improve the CIK cell immunotherapy and extend the utalities of CIK by study activation and regulation of T cell in culture of CIK cell. Methods: Apoptosis rate and molecular marker in membrane surface of T cell analyzed by flow cytometer. The role of various cytokines and immune-regulating factors determined by measuring T cell subpopulation rate. TCR repetoire analyzed by RT-PCR, relative telomere length of CD8+ T cell determined by real-time quantification PCR and the cytotoxicity of CIK cell determined by the death rate of CFSE labeled target cells. Results: 1)The role of IFN-γ contributes to the decresed ratio of CD3+CD4+ cell in CIK. 2)The exist of IFN-γ in CIK culture is responsible to the inhibition of Treg and to the increased ratio of cells differentiating to CD3+CD56+cells.. 3) In vitro cultured CIK cell expressed regular TCR repetoire, that in tumor patients’ PBMC usually showed biased or deficient. Conclusion: T cell in the in vitro cultured CIK could benefit from immune regulation, of which the inhibition of Treg by IFN-γ may be the potential mechanism. In vitro cultured CIK facilitates the recovery of TCR diversity, which may be beneficial to repairing the immune function of tumor patients.