| Objective:The purpose of this study is to detect the expression of peripheral blood regulatory T cell(Treg) and IL-17 producing-helper T lymphocyte(Th17) in cerebral ischemic stroke(IS) patients, and to explore the possible immunological mechanism of high sensitivity c-reactive protein which were increased in patients, to provide new ideas for treatment of immunology ischemic stroke model.Methods: 44 IS patients was collected within 24 hours of onset from 2013 December to 2014 June in Gansu People’s Hospital of neurology(case group), 24 cases of male and 20 cases of female were involved; age ranged from 40 to 70(57.55 + 3.22). 29 age-matched healthy subjects was collected in the same period as a control group in Gansu People’s Hospital Medical Center, 16 cases of male and 13 cases of female were involved, age ranged from 40 to 70(57.55 + 3.22) years old. Exclusion criteria: After a detailed history and head CT or MR examination, intracranial ischemic lesions were revealed; with a history of IS or cerebral hemorrhage complicated with coronary heart disease; the functions of liver and kidney were serious insufficient; peripheral vascular disease or peripheral vascular occlusive diseases; malignant tumor patients and rheumatic disease patients. Infectious diseases such as sepsis, severe infection of the upper respiratory tract, as well as the application of anti-inflammatory drugs(such as non-steroidal anti-inflammatory drugs) were excluded. The expression level of Treg cells, Th17 cells and hs CRP in peripheral blood of each groups were measured by flow cytometry; the correlation analysis of Treg cells, Th17 cells, hs CRP and the degree of neural function defect in the peripheral blood of patients were detected by SPSS17.0 software.Result:1.The level of common stroke risk factors(diabetes, hypertension, hyperlipidemia and high homocysteine levels) in case group, were higher than control group(P < 0.05). In stroke patients average hs CRP was 5.41 ±1.96, higher than the non-stroke group(P<0.000). Group.2. Treg, hs CRP, and Th17 level of peripheral blood in cases group different to control group(P < 0.05).3. Peripheral blood regulatory Treg cells percentage in cases groups with different degree of nerve malfunction lower than the control group, but hs CRP and Th17 cells expression was significantly enhanced(P < 0.05); The severe peripheral regulatory percentage of Treg cells is lower than the moderate group; Moderate peripheral blood percentage of Treg cells is lower than the mild group(P < 0.05). And hs CRP and Th17 cells are characterized by severe peripheral blood hs CRP and Th17 cells express higher than moderate groups, moderate group of peripheral blood hs CRP and Th17 cells express higher than the group(P < 0.05).Conclusion: 1.The increased degree of hs CRP in peripheral blood of patients with acute ischemic stroke have certain relevance with the imbalance expression of Treg/Th17, this indicates that the imbalance expression of Treg/Th17 cells may be a major cause of hs CRP in patients with ischemic stroke. 2. The level of Treg cell in peripheral blood and Th17 cell can become a non-invasive assessment indicator of early cerebral ischemia in acute ischemic stroke patients. 3. In patients with acute ischemic stroke, the level of Treg cell and Th17 cell in the peripheral blood associated with nerve function defect degree, suggesting that it can be used as a sensitive index to reflect the severity of ischemic brain injury... |
PDF Full Text Request