| Object Geranium eriostemon Fisch.ex DC.is a kind of perennial herbs belonging to willow herb geranium, Geraniaceae.systemic research of reports about Geranium eriostemon Fisch.ex DC of heilongjiang province are rare at present.most of them are crude drug identification and medicinal chemistry research, etc.Therefore, this topic take Geranium eriostemon Fisch.ex DC. as the research object, extraction technology designed to lay the foundation for further improvement of mullein Geranium active ingredients, for the development of quality standards, development and utilization of medicinal resources that provide the basis for more rational.Methods Take fresh Geranium eriostemon Fisch.ex DC. as experimental material, Using paraffin section technology, made permanent of root, leaf and peduncle in respectively. then observed under a microscope and photographed.take ultrasonic extraction as method to extract total flavonoids and tannin of Geranium eriostemon Fisch.ex DC. Used spherical symmetry design optimization to get the best extraction of total flavonoid and tannin.Used method of direct drying to determine moisture content of the sample. Used method of burning law to determine ash content of the sample. Used method of UV spectrophotometry to determine mineral elements of the sample. Used method of thermal extraction to extract shikimic acid, Extraction process is 15 times the amount of water, extracted 100 min, at the temperature of 80℃. The extracted liquid was concentrated, by column chromatography to obtain shikimic acid meal, determination of shikimic acid by high performance liquid chromatography, Chromatographic conditions:column was Agilent C18(150 mmx4.6mm,5 um), the mobile phase was 0.1% phosphoric acid-methanol(90:10), the flow rate was 1.0mL.min-1, detection wavelength was 214 nm, the column temperature was 25℃. Used prothrombin kit for shikimic acid sample which was subjected to anti-clotting activity detection.Results Cortical cells of Geranium eriostemon Fisch.ex DC. containing Cubic,calcium oxalate crystals and starch grains.Vascular is the tough type of outside.Cambium is obviously. bifacial leaf, main vein vascular is the tough type of outside.Palisade tissue and spongy tissue were significantly.Epidermis of peduncle with Furry. Cortical to cross-section is about 1/4. Vascular is the tough type of outside and divided into 4 or 5. By spherical symmetry design optimization, analysis indicated the optimum conditions of total flavonoid were 40-fold solvent, 60% ethanoland 35 min for extraction. analysis indicated the optimum conditions of tannin were 24-fold solvent, 20% ethanoland 50 min for extraction. Total flavonoids in different periods of Geranium eriostemon Fisch.ex DC.were:May 1.59%, 1.78% in June, July 1.93%, 2.20% in August, September 1.80%. tannin in different periods of Geranium eriostemon Fisch.ex DC.were:May3.68%, June3.81%, July 4.07%, August2.75%, September2.13%. Ash content of Geranium eriostemon Fisch.ex DC. was 8.67%, moisture content was 8.16%; content of iron was 223.6 ug/g, content of zinc was 445.7 ug/g, content of manganese was 303.9 ug/g, content of Copper was 209.1 ug/g, content of potassium was 868.9 ug/g, content of calcium was 1951.9 ug/g, content of magnesium was 1347. 8 ug/g. Calculated yield of shikimic acid was 44.3%.Conclusions Observation of the different parts of Geranium eriostemon Fisch.ex DC.Vascular of roots, leaves, peduncle are outside the tough type of vascular. Vascular of peduncle was 4 or 5. According to optimum extraction,content of total flavonoids was the highest in August, content of tannin was the highest in July, Therefore,the best time of harvest is july to august.Mineral elements of Geranium eriostemon Fisch.ex DC. are abundant.Anticoagulant activity in vitro studies have shown that shikimate of Geranium eriostemon Fisch.ex DC. has some anticoagulant activity. |
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