Study On The Pre Paration And The Quality Standard Of Com Pound Fufangguangjinqiancao Granules

Com Pound Fufangguangjinqiancao from the clinical ex Perience was consisted of Desmodium Styracifolium, Liquidambaris Fructus, Plantain Seed, Alisma orientalis(Sam) Juze P, Achyranthes bidentata BL, Ventilago leiocar Pa Benth, Glycyrrhiza uralensis Fisch, Malvae Fructus, Dianthi Herba, Astragali Radix, Codono Psis Radix, Poria, Glycyrrhizae Radix Et Rhizoma, which based on the characteristcs of stagnation of dam P-heat, deficiency of s Pleen and stomach, qi stagnation and blood stasia in urinary system stone. It has the effection of removing heat and dam Pness, diuresis and lithagogue, drench recanalization and analgesic, which is mainly a PPlied to urinary system stones.This paper is based on a serious studying of the treatment of urinary calc uli situation, clinical methods, the commonly used traditional Chinese medicine and diagnosis and treatment in traditional Chinese medicine. This to Pic mainly study on Com Pound Fufangguangjinqiancao granules from Pre Paration and qua lity standard based on Pharmacodynamics. Water load test in rats to study diur ctic action and lithagogue test for kidney stone in rats of Fufangguangjinqianca o extractives Processed by different methods. The rat model of renal calcium o xalate stones was induced by administrated ethylene glycol and ammonia chlori de, after 30 days modeled and administered, the incidence of kedney stone, ox alic acid and calaium in kedneys and urinary.Fufangguangjinqiancao extractives Processed by different methods all have effect on diurctic action and restrainin g the formation of kidney stone.The extraction hygrosco Picity from water extraction technology and alohol Preci Pitation Process of water extract of desmodium styracifolium and other fiv e herbs and alcohol extraction technology of alisma orientalis and other five he rbs is small, so this technology ado Pted. Orthogonal test was ado Pted to o Ptimize water extraction technology and alohol Preci Pitation Process of water extrac t of desmodium styracifolium and other five herbs, res Pectively. With com Posit e score of contents of alisol B 23-acetate and total flavanoids, yield of dry ext ract as indexes, alcohol extraction technology of alisma orientalis and other fiv e herbs was o Ptimized by orthogonal test. OPtimun water extraction conditions were as follows:extracted 3 times with 8-fold water, 1.5h each time; OPtimun alcohol Preci Pitation Process of water extract Process was: initial density of w ater extract 1.0 g·ml-1, alcohol Preci Pitation concentration 70%, alcohol sedimen tation time for 8 h; OPtimun alcohol extraction conditions were as follows: ext racted 2 times with alcohol concentration 60%, first time 1h with 8-fold alcoh ol concentration 60%, second time 0.5 h with 6-fold alcohol concentration 60%.When dextrin:lactose:extract Powder:5%PVP dissolved in 95% ethanol was8:2:6:4:5, the effction of granulation is well, the granules a PPearance is good,and Particle size, water, re Pose angle and thawing ossifying Passed the Chine se Pharmaco Poeia requirments.TLC was used to identify Desmodium Styracifolium,Plantain seed,Alisma orientalis(Sam) Juze P, Achyranthes bidentata BL,Ventilago leiocar Pa Benth in Fufangguangjinqiancao granules.The UPLC method was used for schaftoside and 2,3-acetylalisol determination, to establis the standdard of Com Pound Fufa ngguangjingqiancao granules for its safty.The results for this Pa Per shows that the Pre Paration Processing is reasona ble and feasible, the quality is controllable, and the effection is affirrmation, w hich can Provide safety and effectiveness guarantee for clinical use and data fo r new drug develo Pment.