Study On The Sample Preparation Of Traditional Chinese Medicine By Laminar Flow Extraction Microchip

In mid-1990 s, Microfluidic chip is one of micro total analysis system for important area of research in the field of analytical chemistry. Microfluidic chip technology with advantages of high separation efficiency, fast analysis, minimal sample reagent consμmption, ease of integration, miniaturization, automation and so on. The technology is based on the theory of analytical chemistry, with a variety of micro-processing methods, such as micro-motor(micro electrical mechanical system, MEMS) technology to build processing chip structure. Currently, the microfluidic chip technology is widely used in the medical field, biological field, analytics field, food industry field, environmental field, and so on. It is the focus of today’s micro total analysis system development.In this paper, microfluidic liquid-liquid extraction techniques extract the active ingredients of Chinese herbal medicines separation, which is a new method for microfluidic laminar flow continuous liquid-liquid extraction. The main contents of the article are the following four parts:In the first chapter, research explain the progress of microfluidic liquid-liquid extraction technique in the field of traditional Chinese medicine and review development of this technology in the future. And what’is more,we also sμmmarize the implications of liquid-liquid extraction techniques, laminar flow theory and microfluidic liquid-liquid extraction technology.In the second chapter, a technology based on liquid- liquid laminar flow microchip extraction for ginseng sample pretreatment is setting up. By using syringe pμmps, the extract solution and reagent were introduced into the microchip channel respectively to get the ginsenosides extracted. Samples were collected, and then detected with HPLC. Pre-treatment method of the chips, the ratio of the flow rate of the extract solution and reagent which were introduced into the microchip, the velocity of the extract solution and reagent, the width of the channel and other factors are optimized in this experiment. The phase interface is more concentrated, stable and durable when the microchip was washed with pure water and then air-dried before experiment. Extract effect will be much better in the case of extract liquid flow rate V(water saturated n-butanol): V( extract of gensing) = 6:12(μL/min), the microchip channel width for the extract effect is more ideal case of 200μm. Good reproducibility was obtained under the optimμm conditions--- relative standard deviation less than 2%(n = 6). The contents of Rg1, Re, Rb1 by chip extraction were: 1.05,0.54,1.30 mg/m L,in only 2 minutes.Compared with traditional extraction,the rate of chip extraction increased by 12%, 13%, 15 %,respectively. This method is simple, fast, high extract efficiency for ginseng sample pretreatment.In the third Chapter, according to silylation reaction principle, use a syringe pμmp in the extraction side inject silane reagent into the double Y-chip channel, and the dual-channel side of the Y-chip extraction will have this silylation reaction. Ultimately, we get the extract chips with one side of chip channel is hydrophilic and the other side is hydrophobic. Apply this modified double Y chip to do extraction test, and compare the results of extracted results with the unmodified chip extraction, the modified chip was found that the extraction of the modified two-phase interface is more stable and controllable, and higher extraction efficiency. Finally, after successfully improving the double Y-chip slightly modified to optimize conditions for the continuous improvement of Y-chip dual-channel modified to do so to get the character is not the same on both sides of the chip channel continuous double Y extracted chips.In the forth chapter, a continuous double Y-chip was designed, through this type of chip structural features to do continuous extraction, separation, and purification of ginsenosides and chelerythrine. Extraction efficiency as their respective targets, using designing experiment to see the different factors’ influence to extration rate,such as the width of the channel,and the effects of surface modification and the flow rate of extraction stream.Ultimately, excellent level and excellent combination of three factors were come up with the result which was analysed by the poor analysis and the variance analysis. Based on this and microfluidic continuous liquid- sample extration system to build up the method of dealing with the Chinese medicine samples.