Synthesis Of Curcumin-Selenium Coordination Compound And Intervention On Liver Injury Exposed To Cadmium In Rats

Objectives This study aimed to synthesize curcumin-selenium coordination compound from curcumin and sodium selenite(Na2Se O3), to establish the HPLC method to measure this coordination compound, and to investigate its stability and efficacy for cadmium(Cd)-induced liver injury in rats.Methods 1 Synthesis of curcumin-selenium coordination compound: Curcumin-selenium coordination compound was synthesized by reaction between curcumin and Na2 Se O3,purified by column chromatography, and its structure was identified by IR, HPLC-MS and AFS. The HPLC method to determine this compound was established by the assay of uvvisible spectrometry and HPLC. Stability of this coordination compound at different conditions of temperature and light was tested. 2 The effect of curcumin-selenium coordination compound on liver damage of rats exposed to cadmium: Thirty SD rats were randomly divided into five groups of six: a control group, a Cd exposure group, a low-dose curcumin-selenium coordination compound intervention group, a medium-dose curcuminselenium coordination compound intervention group and a high-dose curcumin-selenium coordination compound intervention group. All groups except the control group were given cadmium chloride(Cd Cl2, 2mg/kg, i.p.) every other day for six times. The control group was given the same volume of normal saline. Curcumin-selenium coordination compound was given to the low-dose curcumin-selenium coordination compound intervention group at 1.44mg/kg(i.g.), the medium-dose curcumin-selenium coordination compound intervention group at 2.88mg/kg, and the high-dose curcumin-selenium coordination compound intervention group at 5.76mg/kg, once a day for 6 weeks. The control group and Cd exposure group were given water of the same amount as control. The levels of alanine aminotransferase(ALT), aspartate transaminase(AST), malondialdehyde(MDA),superoxide dismutase(SOD), cysteinyl aspartate specific proteinase 3(Caspase-3) were measured at the end of the experiment. Cd content in liver was determined by inductively coupled plasma mass spectrometry(ICP-MS). And pathological changes in the liver were observed.Results 1 Curcumin-selenium coordination compound was successfully synthesized. The molar ratio of curcumin to Se was 1:1, and the mass fraction of Se was 16.6%. Theparameters of the HPLC method to detect curcumin-selenium coordination compound were:mobile phase: acetonitrile/water/acetic acid=95/4.5/0.5, detection wavelength: 380 nm,temperature: 25 ℃, and flow velocity: 1ml/min. Degradation rate profiles of curcuminselenium coordination compound and curcumin under different conditions of temperature and light were drawn. 2 The levels of ALT, AST, MDA, Caspase-3 in cadmium exposure group were significantly higher than those in control group, and the level of SOD was significantly lower. While the levels of ALT, AST, MDA, Caspase-3 in curcuminselenium coordination compound intervention groups were significantly lower than those in cadmium exposure group, and the level of SOD was significantly higher. Cd content in liver was not significantly reduced by curcumin-selenium coordination compound. Hepatic lobules of the Cd exposure group were severely destroyed and hepatocytes were damaged,or even committed necrosis. The pathological changes were much alleviated in the intervention groups compared to the Cd exposure group.Conclusions 1 Curcumin-selenium coordination compound is successfully synthesized. 2Curcumin-selenium coordination compound has therapeutic effect on liver injury of rats exposed to cadmium.