After The Muscle Contusion Mitochondrial Oxidative Stress-related Activity Changes

Research purposes:The sports injury are relatively common in amateur sports, professional sports training and competition, and skeletal muscle blunt trauma is a main damage type. Skeletal muscle blunt trauma often leads to damaged muscle fiber dissolving, DNA damage, inflammation, scar tissue, skeletal muscle stress drop and so on, severe cases can cause human body decline and even loss of movement ability. In previous studies the research team found that during the skeletal muscle damage self repair process, quality and quantity of mitochondria may be in a certain degree of change in histological Angle, it is also affected to get the expression of aerobic metabolism related enzymes from the perspective of proteomic expression analysis, which could have a certain effect on skeletal muscle damage self repair. Blunt trauma of mitochondrial damage after skeletal muscle injury causes aerobic capacity drop so that the speed of recovery becomes slowly, and the change of mitochondrial oxidative stress status inside play a role on the regulation of mitochondrial function. This experiment have a study on the skeletal muscle mitochondrial oxidative stress status inside after blunt trauma by testing rats calf CK after blunt trauma of mitochondria and SOD, MDA, CAT and gsh-px, LDH, ALP and other indicators of change, the purpose is to analysis a possible link among various indicators, to explore whether it’s beneficial to recover their physical condition after a sports injury, provides a new theoretical basis for the research on the movement’s blunt trauma of the body and its recovery process.Methods of the research:54 Wistar rats, provided by experimental animal center of Shandong University, are divided into three groups : the experimental group, experimental group and control group. After purchase, carry out the adaptive feed for a week in accordance with the standard conditions, use the blunt injury blow device to build the model. After blunt trauma, beheaded kill the rats respectively in 30 days, 15 days, ten days, 7 days, 5 days, 2 days, 12 hours, and get rats calf and extract the mitochondria todetermine indicators. The test kit is target preparation kit built in nanjing, the other instruments and equipment used in the experiment are provided by the experiment center of Shandong institute of physical education and life science college of Shandong University lab, the statistics of data in experiment are analyzed by SPSS20.0 statistical package of social science.Results of the research:1、Alkaline phosphatase(ALP), the activity of ALP increased significantly and reached a peak(P <0.01) in the 12 hours after blunt trauma. Two days later, the activity of ALP decreased, compared with the control group(CON), the difference was extremely significant(P <0.01). Five to thirty days after contusion, the activity of ALP had a recovery trend, compared with the control group(CON), the difference was not significant(P> 0.05).2、Catalase(CAT), the activity of CAT increased significantly and reached a peak(P <0.01) in the 12 hours after blunt trauma. Two days later, the activity of CAT decreased, compared with the control group(CON), the difference was extremely significant(P <0.01). Five to thirty days after contusion, the activity of CAT decreased and had a recovery trend, compared with the control group(CON), the difference was not significant(P> 0.05).3、Glutathione peroxidase(GSH-PX), the activity of GSH-PX increased significantly in the 12 hours after blunt trauma and reached a peak(P <0.01) in the 2 days after blunt trauma. Five days later, the activity of GSH-PX decreased, compared with the control group(CON), the differences of those three cases were extremely significant(P <0.01). 7 to 30 days after contusion, the activity of GSH-PX had a recovery trend, compared with the control group(CON), the difference was not significant(P> 0.05).4、Creatine kinase(CK), the activity of CK increased significantly in the 12 hours after blunt trauma and reached a peak(P <0.01) in the 2 days after blunt trauma. Five days later, the activity of CK decreased, compared with the control group(CON), the differences of those three cases were extremely significant(P <0.01). In the seventhand tenth day after blunt trauma, compared with the control group(CON), enzyme activity has no significant difference(P> 0.05). In the fifteenth and thirtieth days after the contusion, activity increased and the difference was significant(P <0.05) compared with the control group(CON).5 、Superoxide dismutase(SOD), the activity of SOD increased significantly in the 12 hours after blunt trauma and reached a peak in the 2 days after blunt trauma.. 7 to 30 days after contusion, the activity of SOD decreased, but compared with the control group(CON), the difference was significant(P> 0.05).6、Malondialdehyde(MDA), the activity of MDA was stable in the 12 hours after blunt trauma and increased in the 2 days after blunt trauma. Five days later, the activity of MDA increased significantly and reached a peak in the 7 days after blunt trauma, compared with the control group(CON), the differences of those two cases were extremely significant(P <0.01).7 to 30 days after contusion, the activity of SOD decreased, but compared with the control group(CON), the difference was significant(P> 0.05) In the 10 th and 15 th and 30 th days after the contusion, the activity of MDA decreased and the differences of those two cases were significant(P <0.05) compared with the control group(CON).7、Lactate dehydrogenase(LDH), the activity of LDH increased significantly and reached a peak(P <0.01) in the 12 hours after blunt trauma. Two days later, the activity of LDH decreased, compared with the control group(CON), the difference was extremely significant(P <0.01). Five to thirty days after contusion, the activity of LDH had a recovery trend, compared with the control group(CON), the difference was not significant(P> 0.05).Conclusions:1、The activity of ALP and CAT increased at first and decreased then. In 12 hours to 2nd day after contusion, the difference was significantly higher than control group(CON) which indicated that at this stage, the metabolism of active oxygen enhanced and cell membrane was damaged, hydrogen peroxide was an important cause of cell injury.2、The activity of GSH-PX 12h-5d was significantly higher than control group in 12 hours to 5th day after blunt trauma. It suggested that in this stage, mitochondrial hydrogen peroxide and lipid peroxidation were active and cells were in the phase of oxidative stress adaptation.3、The activities of CK and LDH went upward at first and then went downward. In 12 hours and 2nd day after contusion, the activity peaked, but the activity of CK began to decline after the 5th day after contusion and the activity of LDH declined significantly in 2 days after the contusion. It showed that energy-related intracellular enzymes were induced significantly and the permeation of membrane changed significantly.4、The trends of SOD changes indicated the speed of producing superoxide anion radical is the fastest in 12 hours to 2nd day after injury. Then it gradually declined. But, it was still significantly higher than the control group; MDA showed some hysteresis, latency, the content was stable and it did not change significantly after contusion in 12 hours after contusion. enzyme The content of MDA significantly increased in 2 days after contusion and reach a peak in 7 days after contusion. The content decreased in 10 days, which indicated the system of body have a stage of adaptation when suffer a injury.