| Skeletal muscle injury presents the most common injury in sports medicine,whose incidence varies from 10%-55%.Skeletal muscle injury presents a challenging problem in sports medicine,as injured muscles heal very slowly and often with scar recovery, which severe influence motor function.It has been observed that injured muscles can initiate regeneration promptly,but the healing process is often inefficient and hindered by the formation of scar tissue,which may contribute to the tendency for muscle injury to recur.It has been reported that skeletal muscle contusion at early can direct trauma to microvessels results in membrane damage,endothelial cell swelling,perivascular hemorrhage,and uncontrolled clot formation.leading to additional local ischemia,in turn,induces further degradation of membrane phospholipids,accumulation of free radicals,and inflammation-induced oxidative stress may aggravate evolving tissue damage,previous experimental studies have shown that maximum posttraumatic microcirculatory disturbances and secondary tissue damage evolve over time and are not observed before 24 hours after contusion.The presence of local microcirculation damage and inflammation reactive in muscle may lead to overproduction of reactive oxygen species.These species may damage lipid membranes and produce cell necrosis, but antioxidant enzymes such as superoxide dismutase,catalase and glutathione peroxidase could reduce oxidative stress and protect skeletal muscle cell.Thus,early restoration local microcirculations,reduce free radicals,elevate antioxidant enzymes activities are becoming a hot research.Our previous experimental studies have shown that Chinese medicine HuangqiDanshen could promote skeletal muscle expression of myosin heavy chain,short the healing process of injured muscle and enhance regeneration of injured muscle.However the detailed mechanism underlying beneficial effects of Huangqidanshen on skeletal muscle contusion remain to be established. Therefore,the aims of this experimental study are to asses the effects of HuangqiDanshen on oxidative stress and microcirculation after acute skeletal muscle contusion.In the part one of our study,we observe the effect of Chinese medicine HuangqiDanshen on oxidative stress in rat skeletal muscles after acute contusion. The present study aimed to investigate the effect of HuangqiDanshen on parameters of oxidative stress,namely lipid peroxidation product malondialdehyde(MDA) and the activities of antioxidant enzymes,catalase(CAT),superoxide dismutase(SOD) and glutathione peroxidase(GSH-px),in skeletal muscle after contusion.Thus,to establish whether use of HuangqiDanshen could scavenge free radical,elevate antioxidant enzymes activities in the process of skeletal muscle after contusion.In the part two of our study,we observe the effect of Chinese medicine HuangqiDanshen on microcirculation in rat skeletal muscles after acute contusion. This study quantitatively analyzed the change of blood hemodynamics,the edema index of skeletal muscle,the changes of arteriolar internal diameter,microvascular flow rate.Therefore,to observe whether administration HuangqiDanshen could amelioration microcirculation injury,attenuate tissue edema,and promote the healing progress of the contused skeletal muscle. Part One Effects of Chinese Medicine HuangqiDanshen on Oxidative Stress in Rat Skeletal Muscles after Acute ContusionObjective To investigate the effects of Chinese medicine HuangqiDanshen on oxidative stress in rat skeletal muscles after acute contusion..Methods Seventy two male Sprague-Dawley rats were randomly divided into three groups(24 rats each group):(1) Chinese medicine HuangqiDanshen group;(2) physiological saline group;(3) natural healing group.The muscle contusion was produced at the gastrocnemius by specific device;Local injection by Chinese medicine HuangqiDanshen at injured site;group physiological saline treated with the injection of physiological saline,and group natural healing for control.The remaining six rats were used as normal control.The muscles were sampled at the day 1,4,7 and 14 after injury respectively,and then investigated the parameters of oxidative stress,namely MDA and the activities of antioxidant enzymes(CAT,SOD and GSH-px) in gastrocnemius after contusion..Result Compared with the physiological saline group and natural healing group,SOD and GSH-px activities in Chinese medicine group were significantly increase after contusion(P＜0.05).However,the CAT activity had no change(P＞0.05).The concentrations of MDA in Chinese medicine group were lower than that of physiological saline group and natural healing group at days 4,7 after injury(P＜0.05)Conclusion The Chinese medicine HuangqiDanshen could significantly reduce the content of oxidative product MDA,protect the skeletal muscles in the healing process from oxidative injury and markedly increase the reducing enzyme SOD,GSH-px activities of contused skeletal muscle. Part Two Effects of Chinese Medicine HuangqiDanshen on Microcirculation in Rat Skeletal Muscles after Acute ContusionObjective To investigate the effects of Chinese medicine HuangqiDanshen on microcirculation in rat skeletal muscles after acute contusion.Methods Seventy eight male Sprague-Dawley rats were randomly divided into four groups:(1) Chinese medicine treatment group(A);(2) physiological saline group(B); (3) natural healing group(C);(4) control group(D).The acute skeletal muscle contusion model was produced at the right gastrocnemius by specific device.Local injection at the injury site with Chinese medicine HuangqiDanshen in group A;local injection with physiological saline in group B;The group C without intervention at injured site,and the blank group D as control.Microcirculations of gastrocnemius in vivo were observation at 2h,24h,72h and 120h respectively after contusion.Rats that were not injured served as control.Result Compared with the group B and group C,the internal diameters of arteriole in group A were significantly dilation at 24h and 72h,the blood flows of capillary were markedly increase at 24h after contusion(P＜0.05).Compared with group D,the Wet/ Dry Weight Ratios of each group were significantly increase at 2h and 24h,but edema in group A was less pronounced compared with group B or group C.The hemodynamics,however,have no significantly changed in each group(P＞0.05).Conclusions Administration Chinese medicine HuangqiDanshen at early could amelioration microcirculation injury,attenuate tissue edema,and promote the healing process of the contused skeletal muscles.
|
PDF Full Text Request