Effect Of Maternal Lead Exposure On The Calsyntenin-1 Expression In The Hippocampus Of Mouse Offsprings

Objective To explore the effect of maternal lead exposure on the Calsyntenin-1 protein expression in the hippocampus of mouse offsprings. Methods 40 weaning male mouse were divided into four groups. Lead exposure was freely drinking the lead acetate solutions with dosages of 1 g/L, 5 g/L and 10 g/L, respectively, Pb acctate in drinking water from the first day of gestation to postnatal day 21. The control group was drinking deionized water. The lead contents in blood and hippocampus of 21 days hippocampus of mouse offsprings were determined and the protein level of clstn-1 was measured by immunohistochemistry and western blot. Results Compared with the control group, The lead levels in blood and hippocampus of 21 d lead-treated pups were higher than those in the control group(P<0.01). The protein level of Clstn-1 in exposur mmunohistochemistry showed that the Clstn-1 was mainly located in the cytoplasm, The area density of Clstn-1 positive immune reaction in CA1 area of mouse pups in three lead exposure groups were higher than the control group(P<0.01), and the average gray value in lead exposure groups were higher than the control group(P<0.01), The protein level of Clstn-1 in western blot showed that the exposure groups was significantly enhanced compared with the control group(P<0.01). Conclusion Maternal lead exposure upregulated Clstn-1 expression in the hippocampus of mouse offsprings, this might be one of the pathogenic mechanisms of lead neurotoxicity in hippocampus.