| Lung cancer is a malignant tumor and it has higher incidence.The first retrospective survey was carried out in the 1970 s and it shows that lung cancer mortality rate was 5.47 / 100,000,and it is in the fifth place in the cause of cancer death behind gastric cancer, esophageal cancer, liver cancer and cervical caner,it accounts for 7.43% of,all cancer death.Compared with the same period in lung cancer mortality with other countries,it is at a low Alevel.In the early 1990 s,the second cause of death nationwide sample survey results showing that lung cancer mortality was 17.27 / 100,000 and it is at the third place in the cause of cancer death behind gastrc cancer, esophageal cancer.In the top five of cancer spectrum from 2000 to 2007,lung cancer has been in the top spectrum,and 80% of.them are nonsmall cell lung cancer.In 2012, the Office of Cancer Prevention survey shows that China has leapt to the cause of lung cancer mortality in cancer deaths in the first place, accounting for cancer cause of death in 27.93%.As such,understanding of nonsmall cell lung.cancer molecula biology and etiology mechanism would be the key to design targeted therapies and personalized medicine.The development, invasion and metastais of the nonsmall cell lung cancer not only rely on biological proliferative properties,but also rely on absorbing nutrients from the hosting environment.When the tumors reached to 2mm, it need to rely con new blood vessel to provide more nutrients and transport metabolic waste.VEGF plays a very important role in the initiation and regulation of angiogenesis and it also plays ca key role in malignant tumor progression and its prognosis.Vitro and in experiments showing that high levels of VEGFeexpression associated with the tumor growt and metastasis, and by controlling the VEGF signal transduction can inhibit ttumor angiogenesis and ttumor growth itself.However, The findings of relations between VEGF gene single nucleotide polymorphism and lung development remain controversial or uncertain,and there are relatively few reports of the relations between VEGF gene single nucleotide polymorphism and lung cancer development in our country.The purposee of our study is to investigate the VEGF +936C/T polymorphism and its serum levels associated with the NSCLC,and then provide the basis for risk assessment, prevention and early diagnosis and appropriate treatment at the early stage of nonsmall cell lung cancer. Objective:To explore the correlation of the VEGF +936C/T polymorphism andd its serum levels with the NSCLC by testing in patients with NSCLC cancer and normal physical examination in serum VEGF expression levels and their blood cells of VEGF gene loci +936C/T polymorphism. Methods:1 Object of study:(1)Select Chengde Central Hospital of non-small cell lung cancer patients diagnosed pathological diagnosis in September 2010 to May 2012 period 80 cases;and they have no other vascular llesions,a week before the blood they have not undergone any surgery,radiotherapy, chemotherapy, or immuntherapy;it includs 52 males and 28 females, aged from 45 to 72 years,and their median age is 55 years;according to World Health Organization classification of lung cancer: squamous cell ccarcinoma 32 cases, adenocarcinoma 36 cases,larg cell carcinoma 8 cases,adenosquamouss carcinoma 4 cases.The control group are 80 healthy persons who have no other vascular lesions,including 54 males and 26 females, aged 35 to 76 years, their median age is 52.6 years.2 Specimen collection and processing: All subjects met the inclusion criteria were taken venous blood 4ml under fasting state and put them into EDTA tubes.We should centrifug the blood within 1 hour(3000r / min centrifugal 15min),separat serum and put the supernatant into eppendorf tubes, aliquots at-20℃refrigerator. We use the ELISA method for quantitative determination of serum levels of VEGF.We strictly follow Wizard purification of genomic DNA purification kit instructions for DNA extraction from the blood cells precipitate,then We genotype the VEGF gene locus +936C/T(rs3025039) by useing LDR method.3 Statistical methods: we choose SPSS1.7.0 statistical software for all measurement data statistics processing.The measurement data of VEGF serum levels results with x ±s. We use independent samples T-test statistical to analysis the result;We used one-way ANOVA comparing serum levels of VEGF in patients with tumors of different genders and among categories of tumor.We use the genetic equilibrium law to test the distribution of compliance by laws of Hardy-Weinberg equilibrium;We use T tests for continuous variables analyzed data and use the Chi-square test for categorical variables data analysis.We use the Pearsonc2 test methods to test the differences among genotypes. We analyze the relationship between genotype and nonsmall cell lung cancer risk by relative risk and 95% confidence interval.All statistical ttests are two-tailed test, P<0.05 was cconsidered statistically significant.Results:1 NSCLC group are significantly hhigher than the control group for the expression level of VEGF,P=0.001(P<0.05),and the result is a statistically signifficant difference.2 Among differentt tumor classes,there are not significant differences in serum levels of VEGF,P=0.151(P>0.05);among different sexes there are not significant differences inn serum levels of VEGF,P=0.596(P>0.05);show that there are no differences between patients with different categories of tumor and sexs for serum levels of VEGF in this experiment.3 Genotype frequencies(c2= 0.960,0.750<P<0.900, P>0.05) and allele frequencies(c2= 0.968,0.250<P<0.50, P>0.05),they show that there are no statistically significant ddifferences between the two groups in genotype ffrequencies and allel frequencies.The results of correlation between genotype and nonsmall cell lung cancer group(PTC=0.554, PTT=0.670, P> 0.05) were not statistically ssignificant.Conclusion:1 VEGF was overexpressedd in NSCLC,and VEGF have relevant with the ooccurrence and development of non-small cell lung cancer.2 There are no differences among patients with ddifferent categories of tumor and sexs for serum levels of VEGF in this experiment.3 There are no significant correlation between the VEGF +936C/T polymorphism and the occurrence and development of non-small cell lung cancer. |
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