| Objective: To investigate the effects of ketamine on βCa MKII and Glu R1 in habenular and the behavior of depression rats.Methods: Twenty-two adult Wistar-Kyoto(WKY) rats were randomly divided into saline control group(depression group) and ketamine(Ket group)and eleven adult wistar rats(normal group).Rats in Ket group were injected with 10mg/kg ketamine,those in normal group and depression group were administrated with same amount of normal saline for three days.On the fourth day,Sucrose preference test was adopted to measure the degree of sugar preference on rats. On the fifth day,Open field test and forced swimming test were used to explore the behavior of rats. The sections of habenular were obtained at half an hour or the behavior tests, and the expression of βCa MKII and Glu R1 were determinded by Western blotting.Results:When the depression group compared with normal rats, sugar percentage decreased,the number of standing reduced in open field test,the immobility time increased significantly in the forced swimming test,expression levels of Ca MKIIhabenula elevated(P<0.05);while Ket group compared with the depressive rats, sugar percentage rised, the number of standing increased in open field test,the immobility time shortened in the forced swimming, the level of habenula Ca MKII expression was reduced,expression of Glu R1 unchanged.Conclusion: Application of ketamine in depression rats could reduce depressive symptoms, and the mechanism may be associated with the down-regulation of depression of βCa MKII in habenular. |
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