Research On Chemical Constituents Of Litsea Glutinosa And Litsea Garrettii

Two medicinal plants, Litsea glutinosa(Lour.) C.B. Rob., Litsea garrettii Gamble were chemically investigated and their antitumor activities have been evolved. Chromatographic methods such as the positive phase silica gel, reverse phase chromatography, Sephadex LH-20, MCI, HPLC have been used. With methods such as thin-layer chromatography, ultraviolet light detection to identify the purity of the components, the structure of the compounds were determined by ESIMS and NMR. Twenty-seven compounds including one new compounds, two compounds isolated from natural plant for the first time, other compounds were first isolated from the two plants.Twenty two compounds were separated and purified from Litsea garrettii,including benzoic acid(1), phytol(2), 1-(2-Phenylcarbonyloxyacetyl) benzene(3),8-hydroxy-6-methoxy-3-pentylisocoumarin(4), 5-Pentyl-3-methoxy-N-phenylamine(5), Sitosterol(6), ethyl orsellinate(7), N-[1-(benzoyloxy)ethyl] benzamide(8),N-(2-phnethyl) benzamide(9), N-[2-(benzoyloxy)-1-methyl-2-phenylethyl]-benzamide(10), N-[2-(acetyloxy)-1-methyl-2-phenylethyl]-benzamide(11), 5-Hydroxyethyl salicylate(12), isovanillic acid(13), salicyl alcohol(14), 4-Hydroxybenzoic acid(15), syringic acid(16), kaempferol(17), gentisic acid(18),quercetin(19), Daucosterol(20), Kaempferol-3-O-α-L-rhamnoside(21), quercetin-3-O-α-L-rhamnoside(22); N-[1-(benzoyloxy)ethyl] benzamide(8) was a new compound; N-[2-(benzoyloxy)-1-methyl-2-phenylethyl]-benzamide(10), N-[2-(acetyloxy)-1-methyl-2-phenylethyl]-benzamide(11) were isolated from natural plant for the first time. Compounds 1-22 were firstly isolated from this plant.Five compounds were separated and purified from Litsea glutinosa, including Pubinernoid A(23), vomifoliol(24), Atroside(25), 1,2-dihydro-6,8-dimethoxy-7-1-(3,5-dimethoxy-4-hydroxyphenyl)-N1,N2-bis-[2-(4-hydroxyphenyl)ethyl]-2,3-naphth alene dicarboxamide(26), Daucosterol(27). Compounds 1-22 were firstly isolated from this plant.The tumor cell proliferation inhibitory activity of some compounds was tested by MTT method. Results show that, within the scope of 40 μg/ml, the measured compounds showed unconspicuously toxicity to Hep G2 and MBA-MD-231 cell,which laid a foundation for later found that Nrf2 agonist.