Effect Of Butylphthalide And Sodium Chloride Injection On The Endothelial Progenitor Cells Of Peripheral Blood In The Acute Ischemic Stroke Patients

This study is to observe the effect of butylphthalide and sodium chloride injection(NBP-injection) on the endothelial progenitor cells of peripheral blood and the score of nation institutes of health stroke scale of patients with acute ischemic stroke,analyse the correlation between the level of endothelial progenitor cells and the concentration of stromal cell derived factor-1α,and explore the mechanisms of NBP-injection treatment on AIS.Fifty-nine cases with NIHSS≥5 were admitted in 72 hours of symptom onset, and randomly divided into NBP-injection group and placebo group. Forteen days was a course of NBP-injection treatment. Elbow venous blood of patients for detecting the level of EPCs and the plasma concentration of SDF-1α was obtained at days 1, 8 and 15, and NIHSS scores were also finished at the same times. EPCs were marked as CD34+CDl33+KDR+ labeled cells by flow cytometry detection. Plasma concentration of SDF-1α were determined by ELISA.The EPCs level of both groups increased gradually in 14 days. At baselines,the EPCs level of two groups has no obvious difference(P>0.05). At 8 days and 15 days,the EPCs level of NBP-injection group were evidently higher than those of placebo group at the same time points(P<0.05,P<0.01).The plasma concentration of SDF-1α had similar changes to the level of EPCs in two groups. NIHSS scores of two groups were gradually reduced in 14 days. At baselines,NIHSS scores of two groups has no obvious difference(P>0.05). And at 8 and 15 days, NIHSS scores of NBP-injection group was significantly lower than those of placebo group at the same time points(P<0.05,P<0.01). Pearson correlation showed that,at 8 days and 15 days,the level of EPCs in NBP-injection group highly correlated with the SDF-1αconcentration( r=0.747,P<0.01;r=0.811,P<0.01). And there were no adverse effects in the two groups.These results indicaded that NBP-injection treatment on AIS could improve nervous function defect effectively. The mechanism may be that NBP-injection could upgrade the expression of SDF-1α to strengthen the mobilization of EPCs.