The Effects Of P12-LOX On Proliferation And Migration Of Gastric Cancer Cell MKN-28

[Objective] To investigate the possible effects of platelet type 12-lipoxygenase (p12-LOX) on proliferation and migration of gastric cancer cell MKN-28.[Method] RT-PCR method for detection the expression of p12-LOX mRNA level in wild type and gastric carcinoma model mice stomach tissues and primary cultured cells, also detected the expression of p12-LOX mRNA level in normal gastric epithelial cell GES-1 and gastric cancer cell MKN-28. MTT method detected for p12-LOX selective inhibitor baicalein effect on MKN-28 cell proliferation in vitro. Wound Healing test detected baicalein on the MKN-28 effect of cell migration in vitro.[Results]1. RT-PCR assay for detection of expression of p12-LOX results showed that mice gastric carcinoma in gastric tissue of p12-LOX mRNA expression was significantly higher than that of the wild type mice (p<0.01).2. Primary cultured wild-type mice and gastric carcinoma in gastric tissue cells, RT-PCR results showed that mice gastric carcinoma in gastric tissue of p12-LOX mRNA expression was significantly higher than that of the wild type p12-LOX mRNA expression gastric tissue of mice.3. Expression of p12-LOX mRNA in gastric cancer cells have significant differences, including high expression in differentiation of GES-1 cells in the lowest, followed by the differentiation of MKN-28 cells, but low differentiation of MKN-45 cells expressing the highest, so when prompted for expression of p12-LOX mRNA can promote the development of gastric cancer.4. MTT essay for detection of MKN-28 cells proliferation in vitro results showed that of baicalein on 25μM,50μM and 100μM-treated cells at 1,3 and 5 days also can inhibited cell proliferation, and showed a dose-dependent, there was significant difference compared with the control group.5. Wound Healing results showed that baicalein on MKN-28 cells 24h and 48h after treatment, cells significantly shorter transport distance compare with the control group (p<0.05 respectively).[Conclusion]1.p12-LOX overexpressed in gastic cancer tissues and cells.2.p12-LOX selective inhibitor of baicalein inhibited MKN-28 cells proliferation and migration via a time and dose-dependent manner.