| Objective:To study the effects of AngiotensinⅡ(AngⅡ)on accelerating proliferation of fibroblast inwound healing and the difference of AngⅡof different concentrations in different wound,toprovide some experimental evidences for clinic therapy.Methods:1. Animal model: 10 healthy males SD rats,weights (175 200) g, supplied by Shanximedical university were randomly divided into NS,low dose,middle dose,high dose fourgroups,ten rats per group .On the day of scalding, hair on rats'back was depilated. Pentobarbitalsolution was injected into the abdominal cavity for anesthesia before scalding. Then four1.5cmx1.5cm full thickness scalded wounds of each rats were abstained by using a professionalhole puncher.2. Wound treatment methods: NS group, wounds were poulticed with saline; low dosegroup, wounds were poulticed with 1×10-9 mol/ml AngⅡsolution; middle dose group, woundswere poulticed with1×10-8 mol/ml AngⅡsolution; high dose group, wounds were poulticed with1×10-7 mol/ml AngⅡsolution. Gauzes containing medicine and the cover used to prevent waterevaporating were changed once per day .Animals helped themselves to food and water during theperiod of the experiment.3. Test index and methods: Observing the four wounds on the back of rats and calculatingthe wound areas at the 5th day after scalded by method of"hyaline membrane and calculating byMIG2000". Making about 1.0 cm×1.0 cm tissue in each wound into single cell suspension.With PI staining, detecting the proportion of S phase (DNA synthesis phase) fibroblasts by flow-cytometry .4. Statistical analysis: All the data were showed in the form of mean±standard ((x|-)± S)deviation, taking P<0.05 as significance level.ResulResults:1. Calculating wound area: On the 5th day after scalded, the wound area of the high dosegroup (1×10-7 mol / ml) was the smallest, 202.6±0.2.Wound area of NS (normal saline) wasthe largest 215.53±0.28.the difference between them was significant (P <0.05). the wound areasof the other two groups were: 209.56±0.29(the low dose group), 207.03±0.26(the middle dosegroup); the difference of the 4 groups'wound area were significant at the 5th day after scalded(P <0.05).2. Cell cycle in wound tissue: The difference of S phase cells'percentage between the druggroups and the NS group was statistically significant (P<0.05); and the difference of S phasecells'percentage among the three AngⅡgroups was also significantly (P<0.05). AngⅡsped upwound healing and the wounds healed with AngⅡdose-dependently.Conclusion:1. Hydropathic compressing locally with AngⅡdoes not affect artery of body, whichensures safety and feasibility.2. Hydropathic compressing locally with AngⅡcan accelerate the full thickness scaldedwound healing process in rat. The reason being hydropathic compressing topically with AngⅡcan increase the differentiation and proliferation of fibroblast in wound tissue.3. Hydropathic compressing locally with AngⅡcan improve wound healing quality.4.The effect is the best when the concentration of AngⅡis 1×10-7 mol/ml. There is arelation of dosage and effect in hydropathic compressing locally with AngⅡ.
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