Detection Of Urine Residues In Occupational Farm Workers After Exposure To Pesticides In Yuanmou County, Yunnan Province

With the development of agricultural economy, pesticide has become an irreplaceable means of production in modern agriculture and has contributed greatly in increasing the agricultural production. However, it also did a lot of damages to the ecological environment as well as human health because usage of some kinds of pesticide which is high toxicity, high amount of residue and poor degradation.Pesticides are playing an increasingly important role in economic growth with development of scientific technology and increase of demonstration base of the production of green vegetables. Meanwhile, residential children, farmers and base workers are exposed in dangerous environment of agricultural production. The residue pesticides in their bodies could be potential threat to their health.Objective:The objective of this research is to conduct experimental analysis of residue in urine of residential children, base workers and farmers using Ultra High Pressure Liquid Chromatograph Coupled with Tandem Mass Spectrometer, UHPLC-MS", and the relation between pesticides and health with statistical data were explored, which further provides statistic referrence and methodology for occupational hazard evaluation.Methods:Analysis and investigation of the urine residue was conducted by the combination of questionnaire and UHPLC-MS". Eluate of urine sepatated from Thermo C18 column (4.6 mm×150 mm,5μm), was introduced from ESI to mass spectrometer. Scanned area of ion trap was as followed:m/z was 50-1500 under positive ion mode; dry airflow was 10.01/min; dry temperature was 500℃; System of high performance liquid chromatography was using Thermo C18 column (4.6 mm×150 mm,5μm); column temperature was 30℃; gradient eluted program comprised of solvent B and A,7 gradients eluted; mobile phase B was 0.01% formic acid acetonitrile solution (V/V); mobile phase A was 0.01% formic acid solution; eluting time was 30 min and flow rate was 0.5 ml/min. All the samples were filtered (0.22 m) before UHPLC-MS" analysis, and sample size was 101. UHPLC-MS" control system was completed by software Analyst 1.6.2. and data processing were using software SPSS 17.0.Results:Urine of 120 people of Yuanmou County were detected and the positive rate of pesticide was 43.3%, among which 7 were children, whose urine residue consists! 1.9%,24 were farmers, whose urine residue consists 54.5%, and 21 were base workers, whose urine residue consists 21.9%.68 urine samples were detected not having pesticide residue, consisting 56.6%.18 kinds of commonly-used pesticides were analysed and 5 were detected in human urine. Carbendazim detective rate in urine is the highest, which is 36.7%; dimethomorph detective rate in urine is 26.7%; Emamectin detective rate in urine is 10%; emamectin benzoate detective rate in urine is 5.7%; and Phorate-sulfone detective rate in urine is 0.8%. Carbendazim residue is obviously the highest residue in human (P<0.05). The variety in urine pesticide residue of base laborers was higher than children and farmers.Through comparison of people who were exposed in pesticides or not, is was observed that urine pesticide residue in people who were exposed is obviously higher than people who were not, and carbendazim and dimethomorph in urine reside of exposure group were obviously higher than control group. (P<0.05). The comparison of positive rate of urine pesticide residue between exposure group and control group in farmers showed that, positive rate of urine pesticide residue of of exposure group is obviously higher than control group(P<0.05). The comparison of positive rate of urine pesticide residue between exposure group and control group in base workers showed that positive rate of urine pesticide residue of workers in exposure group is obviously higher than base laborers in control group.Analysis of the relation between urine pesticides residue and sexuality suggested that there were no statistical relation between them (P>0.05).Conclusion:The research on pesticide residues of human was conducted qualitatively, and an approach to detect human urine using UHPLC-MSn was explored. The results is specified to each sample and therefore reached the expected goal, the evidence of pesticide residue inside the body was observed, and the comparison of relation of urine pesticide residue among different groups of people were conducted, which provided further experimental foundation of technology concerning detecting human pesticide residues precisely and also provided basis to explore pesticide and occupational exposure risk assessment and environmental impact.