| Objective:This experiment by using flow cytometry instrument, through the detection of early cervical cancer and abdominal laparoscopic cervical cancer radical peripheral blood and peritoneal fluid in patients with Th1/Th2 cytokines (IL-2, IL-4, IL-6, IL-10, TNF-a, IFN-r), And through to the follow-up, in patients with proven different surgical methods on the body’s systemic immune and local immune, the influence of for the choice of surgical procedure and postoperative outcomes for patients with early cervical cancer provide theoretical basis.Materials and methods:1.Collect 60 patients with early (Ⅰ A2～ⅡAl) cervical cancer from the third affiliated hospital of kunming medical university (early group-Ⅱal period) of. A randomized.abdominal,30 cases of patients with cervical cancer radical laparoscopic and 30 cases of cervical cancer radical.2. Collection of two groups of subjects before surgery and postoperative 1 day,5 days and 28 days after elbow venous blood 5 ml, And two groups of subjects before surgery and postoperative 1 day,5 days after peritoneal fluid 2 ml, Using flow cytometry instrument detection of peripheral blood and peritoneal fluid Th1/Th2 cytokines (IL-2, IL-4, IL-6, IL-10, TNF-a, IFN-r), Compare the preoperative and postoperative abdominal and laparoscopic cervical cancer radical 1 day,5 days postoperatively, and the change of immune status of patients with postoperative 28 days. Detailed records of two groups of subjects in clinical related information,su.ch as age, weight, operation time, blood loss, aeration time, pathological differentiation, muscular infiltration depth, lymph node metastasis, postoperative complications, and so on.3.Statistical processing All data were statistically processed by SPSS17.0 software.Index of laparotomy and laparoscopic group of measurement data with mean +/-standard deviation (X+S), said the immune index group in comparison with variance analysis, comparison between the two groups use independent sample t-test;The comparison of rate using X2 test.P< 0.05difference was statistically significant,P< 0.001 showed obvious difference.Results:1. Compare ARH group and LRH group before and after surgery in patients with peripheral blood IL-2, IL-4, IL-6, IL-10, TNF-a, IFN-r expression level changes.Two groups of subjects before surgery and postoperative day 28 indicators compared with no significant statistical difference (P> 0.05);Two groups of experimental subjects after 1 day of IL-2, IFN-r expression than preoperative decline, IL-6 and TNF-a expression of preoperative elevated obviously, differences were statistically significant (P< 0.05), and ARH group and IL-6, IL-2 loss TNF-a higher degree than LRH group;ARH IL-6,5 days after the expression of TNF-a more postoperative 1 day decreased (P< 0.05), while the expression of IL-2, IFN-r, there was no significant difference compared with preoperative;Laparoscopic group of IL-25 days postoperatively, IL-6, TNF-a, IFN-r express the preoperative no statistical difference (P> 0.05);Two groups of subjects before surgery, postoperative 1 day,5 days after IL-4, IL-10 expression level had no significant statistical difference (P> 0.05). ARH group blood Th1/Th2 (IFN-y/IL-4) ratiopostoperative day 1 decline, compared with the preoperative (P< 0.05), LRH group blood Th1/Th2 (IFN-γ/IL-4) ratiopostoperative day 1 decline, compared with the preoperative (P>0.05), Two groups of patients after the first five days the ratio recovered to the preoperative level, The postoperative 28 days the ratio was higher than the preoperative level (P<0.05).2. ARH group of patients with postoperative 1 day and 5 days after IL-2, IL-4, IL-6, IL-10, TNF-a, IFN-r expression than preoperative rise, change was statistically significant (P< 0.05); Five days after the expression of IL-2 and IFN-r more postoperative 1 day increased (P< 0.05), The expression of IL-4、IL-6、IL-10、 TNF-a 5 days postoperatively were compared with 1 day postoperative there was no significant difference (P> 0.05). LRH group of patients of postoperative 1 day IL-4, IL-6, IL-10, IFN-r expression than preoperative elevated significantly (P< 0.05); The expression of IL-4,IL-6、IL-10、TNF-a 5 days postoperatively compared with postoperative 1 day significantly decreased (P< 0.05); After 5 days there were no statistical difference compared with preoperative IL-2、IL-4、IL-10、TNF-a、IFN-r expression (P> 0.05);Before the operation, postoperative 1 day,5 days after IL-6 expression level is on the rise (P< 0.05).Two groups were compared ARH postoperative 1 day to 5 days after IL-2, IL-4, IL-6, IL-10, TNF-a, IFN-r rise were greater than LRH group (P< 0.05).Two groups of peritoneal fluid Thl/Th2 (IFN-y/IL-4) ratio decreased on postoperative day 1 than in the operation (P<0.05), the first five days in both groups picked up after the first day, but ARH group was significantly lower than the preoperative level (P<0.05). Five days after is rise Compared with the first day in both groups,but ARH was significantly lower than preoperative level (P<0.05).Conclusions:1. Two groups of patients before and after surgery Cytokines and Th1/Th2 (IFN-γ/ IL-4) value in peripheral blood were compared, laparoscopic radical hysterectomy on systemic immune system interference than traditional open small.2. Two groups of patients Cytokines and Th1/Th2 (IFN-γ/IL-4) value in perioneal fluid were compared, laparoscopic radical hysterectomy than the traditional open surgery for little effect on the peritoneal local immune function and peritoneal local inflammatory lesions. Worthy of clinical promotion.3. Surgical treatment may improve the immune status of patients with early cervical cancer. |
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