Expression Patterns Of Oncomodulin In The Development And Gender Differences Of Mice Brain

Study backgroundDevelopment of the brain is a long process. From the very beginning to the end of life, this process is influenced by many factors, including gene, molecular, environment, etc. The structure and functions of a brain are complicated, with certain differences in sex. Despite some breakthroughs in the study of brain, countless mysteries still remain to be explored and discovered, and many diseases of brain anamorphosis and nerve degeneration depend on these explorations and discoveries to be treated and cured. Currently, brain development study has become the focus of nerve-related science.Calcium binding protein(Ca BP) is an important carrier of the calcium ion in biological signal. Studies show that Ca BP’s family members are found in brains and gather in neurons, and that CaBP plays a significant role in the maintenance of neuron structure and functions, the release of neurotransmitter, and the development of neurons. Oncomodulin(Ocm) is one of the CaBP family members. Researchers have little knowledge, which is mainly confined to inner ear and optic nerve, of Ocm’s representation and function in neuro-system. Some researchers have tested the mRNA level of Ocm in mice diencephalon, finding that Ocm has low representation in mice diencephalon, but whether it has representation in other parts of the brain in unclear. Reports about the representation difference in different developing stages and in different sexes cannot be found either.In this study, we observed the representation and distribution of Ocm in adult mice’s brains. Then we studied the change of its representation at different developing stages. Finally, we observed the different representation of Ocm protein in different sexes. This study lays a morphology foundation of further study of Ocm’s functions.Study methodsWe use the immunity chemistry method of ammonium nickel sulfate’s color strengthening function to test the distribution of Ocm in adult C57BL/6 mice brain and the difference in different sexes. We also use RT-qPCR method to test the representation pattern of Ocm gene at different developing stages(P3d, P7 d, P21 d, P60d) of the mice brain.Study results1. The result of coloring Ocm positive cell is blue-black; the positive production mainly lies in cell cytoplasm, and is mainly represented in neurons.2. Ocm immunity positive production is widely represented in brains, but not equally distributed, with strong positive representation in pallium, hippocampus, thalamus and rare representation in the substantia nigra and striatum3. At different developing stages(P3, P7, P21, P60), Ocm has representation in apophysis mamillaris, pallium, midbrain and cerebella, and the representations are different in different brain parts.4. The representations of Ocm immunity positive production in male and female mice brains are similar. However, the representations of a few nucleus groups are different in different sexes.Conclusions1. Ocm has wide representations in nucleus groups of normal C57/BL6 mice’s brains, which suggests that Ocm is probably involved in some physical functioning.2. Representation of Ocm can be tested in mice brain at different ages, which suggests that Ocm may be involved in the development of brain.3. Ocm protein shows differences in sexes in locating brain nucleus groups, which suggests that although in the same nucleus group, Ocm may play different roles in adjusting in the brain due to different sexes.In short, our results investigated that the expression pattern of Ocm in the brain of mice and its dynamic change in development process,To explore the Ocm in brain function provides an important experimental basis.