The Different Expression Of TLR4and IL-6in NAFLD Mice Induced By High Fat Diets Between Male And Female

Objective: To investigate the different expression between male andfemale NAFLD Kunming mice induced by high fat diets of TLR4and IL-6.Methods: Totally120Kunming mice of half gender were randomly dividedinto normal control groups and model groups, each having30male and30female mice and were fed with normal diet and high fat diet. Every10male and10female mice of each group were randomly selected to execute at8,12and16weeks. The appearance and pathological changes of liver was observed，liverindex, weight difference were calculated, and TLR4, IL-6, TG, Tch and ALTwere determined. Results: The results of pathological examination showed thatthe female mice of model group had no or lesser extent steatosis，the malegenerally had higher degree，and the third batch were more serious than previoustwo batches (P <0.05).Comparing with the same batch male mice,the femalemice have lighter steatosis (P <0.05).The results of examination showed thatweight difference, liver weight, TG, Tch, ALT, IL-6, TLR4shows that comparing with female mice, the male mice were higher (p <0.05), except thatthe compering between the first batch male and female mice of IL-6have nostatistical significance.Comparing with control group, the model group werehigher (P <0.05), except for the TLR4expression detected by RT-PCR of thefirst batch of female mice. And the longer the model group were fed with highfat diet, the higher the detection indexes were (P <0.05). Conclusions: Theexpression of TLR4å'ŒIL-6in NAFLD mice induced by high fat diets werehigher than normal control group, and male mice rose more.It would be better tochoose male mice to build NAFLD model, especial to study NASH.