Buqihuoxue Square Regulation Oxidative Stress And Breast Cancer Patients In Clinical And Experimental Studies To Improve Cardiac Toxicity Of Chemotherapy

Objective:a side by observing Buqihuoxue breast cancer patients to alleviate the clinical efficacy of chemotherapy cardiotoxicity, try to analyze the mechanism.2. Buqihuoxue square adriamycin role in H9C2 cells of rats, suggesting that the protective effect of doxorubicin cardiotoxicity.3, Buqihuoxue side effect of adriamycin on human breast cancer MDA-MB-231 cell lines, indicating that the anti-tumor effect of doxorubicin on impact.Method:1. enrolled 28 patients, all from the Jiangsu Provincial Hospital Breast Diseases (both anthracycline-containing chemotherapy regimens), and randomly divided into experimental group and control group, the experimental group,16 cases,12 cases in the control group, In the control group, postoperative chemotherapy alone; the experimental group on the basis of chemotherapy plus Chinese herb Buqihuoxue side, the two groups before chemotherapy, after chemotherapy, four months after the end of chemotherapy detection of cardiac function (ECG, echocardiography, myocardial enzymes., serum BNP, troponin (CTNT/I)), clinical symptoms of patients, two conventional rat H9C2 cell culture, the cells were treated Buqihuoxue side, to detect cell proliferation rate, and then divided into two groups:Chinese+Table doxorubicin group, epirubicin group. Cell proliferation rate were observed at different drug concentrations, flow cytometry apoptosis, and to detect the expression of VEGF in both groups. 3, routinely cultured human breast cancer MDA-MB-231 cell lines, cells were treated Buqihuoxue side, to detect cell proliferation rate, and then divided into two groups:Chinese+ epirubicin group, epirubicin group. Cell proliferation rate were observed at different drug concentrations, H2O2-treated cells, was detected on two cells produce ROS, analyze its mechanism.Results:1 ratio of arrhythmia treatment group was significantly lower than the control group (p<0.05) 2, the experimental group in echocardiography in EF values than the control group improved significantly (p<0.05), on behalf of certain cardiac diastolic function E/A somewhat reduced after chemotherapy, Buqihuoxue side of diastolic function also has some improvement (p<0.05) 3, the BNP, the experimental group also rose after BNP reduce chemotherapy-induced improvement. (P<0.05) 4, Buqihuoxue parties significantly improved symptoms in patients with chemotherapy appeared include chest tightness, palpitations, fatigue, and nausea. (P<0.05) 2. Buqihuoxue party on H9C2 rat promote value-added effect (p<0.05), can improve cardiac injury (p<0.05) doxorubicin induced apoptosis have to reduce the role of (p <0.05), for its role in promoting VEGF has produced (p<0.05).3, Buqihuoxue side of human breast cancer MDA-MB-231 cell lines inhibit the growth of (p<0.05), Buqihuoxue in inhibiting ROS generation, ros generation of doxorubicin is also effective (p<0.05).Conclusion:1. Buqihuoxue before chemotherapy in breast cancer patients by improving blood rheology, enzymes, oxidative stress reduce the toxicity of chemotherapy drugs on the heart and blood.2. Cell Experimental results show cardiotoxicity of doxorubicin Buqihuoxue side produced a protective effect, the recipe did not affect the anti-tumor effect of doxorubicin.